Brønstad G O, Christoffersen T, Johansen E J, Oye I
Br J Cancer. 1978 Dec;38(6):737-44. doi: 10.1038/bjc.1978.281.
The formation of cyclic AMP was studied in normal liver, subcutaneous hepatomas derived from MH1C1 cells, and premalignant liver and primary hepatomas induced by the carcinogens 2-acetylaminofluorene (AAF) and 4-dimethylamino-azobenzene (DAB). While only very slight effects of prostaglandins (PG) were seen in slices of normal liver, all the hepatomas responded strongly to PGE1 and PGE2. The hepatomas also had increase PGE1-sensitive adenylate-cyclase activity. PGF1alpha and PGF2alpha did not increase the cAMP level significantly either in the liver or in the hepatomas. During AAF carcinogenesis the response to PGE1 increased slightly during the carcinogen feeding, and was greatly elevated only in the fully developed hepatomas. This is in contrast to the increase in adrenalin response seen during carcinogenesis, which starts much earlier, and reaches a peak value within 8--10 weeks. It is concluded that various hepatomas have elevated responsiveness to PGE1 and PGE2 as well as to adrenalin, but the course of change in the tissues' ability to respond to these agents during carcinogenesis is very different.
对正常肝脏、源自MH1C1细胞的皮下肝癌、由致癌物2-乙酰氨基芴（AAF）和4-二甲基氨基偶氮苯（DAB）诱导产生的癌前肝脏和原发性肝癌中环状AMP的形成进行了研究。在正常肝脏切片中，仅观察到前列腺素（PG）的非常轻微的作用，而所有肝癌对PGE1和PGE2均有强烈反应。肝癌中对PGE1敏感的腺苷酸环化酶活性也有所增加。PGF1α和PGF2α在肝脏或肝癌中均未显著提高cAMP水平。在AAF致癌过程中，在给予致癌物期间对PGE1的反应略有增加，仅在完全形成的肝癌中大幅升高。这与致癌过程中观察到的肾上腺素反应增加形成对比，后者开始得更早，并在8-10周内达到峰值。结论是，各种肝癌对PGE1、PGE2以及肾上腺素的反应性均升高，但在致癌过程中组织对这些物质反应能力的变化过程非常不同。
