Department of Oral & Maxillofacial Plastic Surgery, University of Bonn, Germany.
Cancer Invest. 2011 Aug;29(7):478-84. doi: 10.3109/07357907.2011.597813.
The objective of this study was the correlation of Doc-1- and S100A7-gene expression in common oral lesions with their cancerous-transformation risk. Biopsies (n = 15 each) of healthy gingiva, irritation fibromas, leukoplakias and Oral squamous cell carcinoma (OSCCs) were obtained, and after RNA-extraction, transcripts of Doc-1 and S100A7 were quantified by RT-PCR. In comparison with the healthy gingiva, the expression of Doc-1 was decreased, whereas the expression of S100A7 was upregulated in all lesions. As the extent of Doc-1-inactivation and S100A7-overexpression is correlated with their biological behavior, the combined investigation of both genes could be a promising marker in intraoral lesions to estimate the risk for their malignant transformation.
本研究的目的是探讨常见口腔病变中 Doc-1 和 S100A7 基因表达与癌变风险的相关性。我们采集了 15 例健康牙龈、刺激纤维瘤、白斑和口腔鳞状细胞癌（OSCC）的活检组织，提取 RNA 后，通过 RT-PCR 定量检测 Doc-1 和 S100A7 的转录本。与健康牙龈相比，Doc-1 的表达降低，而所有病变中 S100A7 的表达上调。由于 Doc-1 失活和 S100A7 过表达的程度与其生物学行为相关，因此联合研究这两个基因可能是评估口腔内病变恶性转化风险的有前途的标志物。
