Kageha Sheila, Lihana Raphael W, Okoth Vincent, Mwau Matilu, Okoth Fredrick A, Songok Elijah M, Ngaira Jane M, Khamadi Samoel A
Center for Virus Research, Kenya Medical Research Institute, Nairobi, Kenya.
AIDS Res Hum Retroviruses. 2012 Feb;28(2):228-31. doi: 10.1089/aid.2011.0089. Epub 2011 Jul 8.
Human immunodeficiency virus 1 (HIV-1) infection is characterized by genetic diversity such that specific viral subtypes are predominant in specific geographic areas. To determine circulating subtypes of HIV-1 in different parts of central Kenya, a cross-sectional study was carried out on HIV-1-positive blood samples collected from consenting individuals in eight hospitals of Kenya's central province. Proviral DNA was extracted from peripheral blood mononuclear cells. Polymerase chain reaction and direct sequencing using primers generated from a highly conserved region of HIV-1 env gp41 were carried out. Ninety-six samples were successfully amplified and sequenced. Analysis of the sequences showed that a majority of them belonged to subtype A1 (67/96, 69.8%), followed by subtypes D (18, 18.7%) and C (11/96, 11.5%). Consistent with findings in other parts of Kenya, HIV-1 subtype A1 was the most dominant virus in circulation. Continued surveillance of circulating subtypes of HIV-1 in Kenya is important in determining the evolution of the HIV/AIDS epidemic in Kenya.
人类免疫缺陷病毒1型（HIV-1）感染的特点是基因多样性，因此特定的病毒亚型在特定地理区域占主导地位。为了确定肯尼亚中部不同地区HIV-1的流行亚型，对从肯尼亚中部省份八家医院的同意参与研究的个体采集的HIV-1阳性血样进行了一项横断面研究。从外周血单核细胞中提取前病毒DNA。使用从HIV-1 env gp41高度保守区域生成的引物进行聚合酶链反应和直接测序。96个样本成功扩增并测序。序列分析表明，其中大多数属于A1亚型（6七十二分之九十六，69.8%），其次是D亚型（18个，18.7%）和C亚型（11/96，11.5%）。与肯尼亚其他地区的研究结果一致，HIV-1 A1亚型是最主要的流行病毒。持续监测肯尼亚HIV-1的流行亚型对于确定肯尼亚HIV/AIDS疫情的演变非常重要。
