National Center of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan.
J Hazard Mater. 2011 Sep 15;192(3):1132-9. doi: 10.1016/j.jhazmat.2011.06.017. Epub 2011 Jun 23.
The phase-separation phenomenon of non-ionic surfactants occurring in aqueous solution was used for the extraction of lead (Pb(2+)) from digested blood samples after simultaneous complexation with ammonium pyrrolidinedithiocarbamate (APDC) and diethyldithiocarbamate (DDTC) separately. The complexed analyte was quantitatively extracted with octylphenoxypolyethoxyethanol (Triton X-114). The multivariate strategy was applied to estimate the optimum values of experimental factors. Acidic ethanol was added to the surfactant-rich phase prior to its analysis by flame atomic absorption spectrometer (FAAS). The detection limit value of Pb(2+) for the preconcentration of 10 mL of acid digested blood sample was 1.14 μg L(-1). The accuracy of the proposed methods was assessed by analyzing certified reference material (whole blood). Under the optimized conditions of both CPE methods, 10 mL of Pb(2+) standards (10 μg L(-1)) complexed with APDC and DDTC, permitted the enhancement factors of 56 and 42, respectively. The proposed method was used for determination of Pb(2+) in blood samples of children with kidney disorders and healthy controls.
非离子型表面活性剂在水溶液中发生的相分离现象被用于从消化后的血样中萃取与氨 基吡咯烷二硫代氨基甲酸盐 (APDC) 和二乙基二硫代氨基甲酸盐 (DDTC) 分别络合的铅 (Pb(2+))。络合的分析物被定量萃取到辛基苯氧聚乙氧基乙醇 (Triton X-114) 中。多元策略被应用于估算实验因素的最佳值。在使用火焰原子吸收光谱仪 (FAAS) 分析之前，将酸性乙醇加入到富含表面活性剂的相中。对于 10 mL 酸消化血样的预浓缩，Pb(2+) 的检测限为 1.14 μg L(-1)。通过分析认证参考物质 (全血) 来评估所提出方法的准确性。在两种 CPE 方法的最佳条件下，分别络合 APDC 和 DDTC 的 10 mL Pb(2+) 标准溶液（10 μg L(-1)），允许增强因子分别为 56 和 42。该方法用于测定肾病患儿和健康对照者的血样中的 Pb(2+)。
