采用浊点萃取法测定具有不同生理后果的儿童生物样本中的铅。
Determination of lead in biological samples of children with different physiological consequences using cloud point extraction method.
机构信息
Faculty of Science, Department of Chemistry, Erciyes University, 38039 Kayseri, Turkey.
出版信息
Biol Trace Elem Res. 2013 Jun;153(1-3):134-40. doi: 10.1007/s12011-013-9677-9. Epub 2013 Apr 28.
In present study, lead (Pb) level in biological samples of children with physiological disorders (liver, bone, and gastrointestinal; age ranged 1-10 years) have been assessed. For comparison purpose, age-matched healthy children were also selected. Cloud point extraction (CPE) was employed for preconcentration of Pb in acid-digested biological samples prior to its determination by flame atomic absorption spectrometry (FAAS). Dithizone (diphenylthiocarbazone) and nonionic surfactant Triton X-114 (TX-114) were used as complexing reagent and extractant, respectively. The effects of several experimental variables on proposed CPE were evaluated. Under the optimum experimental conditions, the observed detection limit (LOD) and the enhancement factor (EF) were 0.08 μg L(-1) and 53, respectively. Relative standard deviation (RSD) of 10 μg L(-1) Pb was 3.4 %. It was observed that children with liver-, bone-, and gastrointestinal-related disorders had three- to fourfold higher Pb level in blood and scalp hair samples.
在本研究中，评估了生理紊乱（肝脏、骨骼和胃肠道；年龄 1-10 岁）儿童生物样本中的铅（Pb）水平。为了比较目的，还选择了年龄匹配的健康儿童。在火焰原子吸收光谱法（FAAS）测定之前，采用浊点萃取（CPE）对酸消解生物样本中的 Pb 进行预浓缩。二硫腙（二苯基硫代卡巴腙）和非离子表面活性剂 Triton X-114（TX-114）分别用作络合剂和萃取剂。评估了几个实验变量对所提出的 CPE 的影响。在最佳实验条件下，观察到的检测限（LOD）和增强因子（EF）分别为 0.08μg L(-1)和 53。10μg L(-1) Pb 的相对标准偏差（RSD）为 3.4%。结果表明，肝脏、骨骼和胃肠道相关疾病的儿童血液和头皮头发样本中的 Pb 水平高出三到四倍。
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