Laboratory of Pharmaceutical Technology, Ghent University, and Department of Ophthalmology, Ghent University Hospital, Harelbekestraat 72, B-9000 Ghent, Belgium.
Toxicol In Vitro. 2011 Dec;25(8):1919-25. doi: 10.1016/j.tiv.2011.06.009. Epub 2011 Jun 28.
In this research, the slug mucosal irritation (SMI) assay was applied to predict ocular discomfort caused by shampoos to investigate the correlation between responses in slugs and humans. Several SMI experiments and a human eye irritation test (HEIT) were performed with 1 artificial tear solution (ArtTear) and 5 shampoos (A-E; 5%-dilution). In the HEIT, evaluation was performed by participants and an ophthalmologist at several time points. Analyses reveal that (1) a significant positive association existed between immediate stinging reaction reported by the participants and the mean total mucus produced by the slugs (MTMP) (Spearman's Rank correlation=0.986, p<0.001); (2) ArtTear was best tolerated in both tests; (3) moreover, all shampoos induced higher reactions than ArtTear and water; (4) Shampoo A induced the highest MTMP and received higher scores for immediate discomfort; (5) B was the best tolerated shampoo in both tests, while C, D and E resulted in more pronounced reactions; (6) lacrimation was found not to be statistically correlated with discomfort sensations reported by the participants. The SMI assay is a promising evaluation method for discomfort in the human eye. Screening prototype (eye) formulations with this assay allows formula optimization prior to a HEIT.
在这项研究中，我们应用鼻涕虫黏膜刺激（SMI）试验来预测洗发水引起的眼部不适，以研究鼻涕虫和人类之间的反应相关性。我们进行了几项 SMI 试验和一项人类眼部刺激性试验（HEIT），使用了 1 种人工泪液（ArtTear）和 5 种洗发水（A-E；5%稀释）。在 HEIT 中，参与者和眼科医生在几个时间点进行了评估。分析表明：（1）参与者立即刺痛反应与鼻涕虫产生的总黏液量（MTMP）之间存在显著的正相关关系（Spearman 秩相关系数=0.986，p<0.001）；（2）ArtTear 在两项试验中均具有最佳耐受性；（3）此外，所有洗发水引起的反应均高于 ArtTear 和水；（4）洗发水 A 引起的 MTMP 最高，立即不适感得分最高；（5）洗发水 B 在两项试验中均具有最佳耐受性，而洗发水 C、D 和 E 引起的反应更明显；（6）流泪与参与者报告的不适感之间未发现统计学相关性。SMI 试验是一种有前途的人类眼部不适评估方法。使用该试验对原型（眼部）配方进行筛选，可以在 HEIT 之前进行配方优化。
