HLA associations with multiple sclerosis in Greece.

BACKGROUND

Multiple sclerosis (MS) is a demyelinating inflammatory disease of the central nervous system originated by a complex interplay of environmental and genetic factors. The association of MS with the human leukocyte antigen (HLA) class II alleles was investigated in MS patients in northwest Greece, in the geographical region of Epirus.

OBJECTIVE

Our aim was to estimate the prevalence of the HLA-DRB11501, HLA-DQB10602 and HLA-DQA1*0102 alleles, consisting the most common susceptibility haplotype in North European and North American Caucasians.

METHODS

We studied 126 MS patients and 93 age and sex matched healthy controls. HLA typing was performed by a polymerase chain reaction (PCR) amplification with sequence-specific primers (PCR-SSP) method.

RESULTS

We found that HLA-DRB11501, HLA-DQB10602 and HLA-DQA10102 alleles were significantly more frequent among patients (34% versus 11%, p=0.00015; 69% versus 51%, p=0.01; 76% versus 55%, p=0.002, respectively). HLA-DRB11501, HLA-DQB10602, HLA-DQA10102 haplotype was significantly more common among patients (p=0.00067). HLA-DRB11501 and HLA-DQB10602 alleles were more frequently detected in patients with initial symptoms from the brainstem or the cerebellum (p=0.024). No significant correlation was observed among these alleles with sex, disease clinical course, or age at onset.

CONCLUSION

This is the first study to investigate genetic susceptibility to MS in Greece. Our results are in line with previous reports in North European and North American patients.