Department of Periodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
J Periodontal Res. 2013 Aug;48(4):533-9. doi: 10.1111/jre.12043. Epub 2013 Jan 14.
Genetic backgrounds play a key role in susceptibility to and protection against a spectrum of periodontal diseases. Like other infectious diseases, the human leukocyte antigen (HLA) have been found to be associated with periodontitis. This study aimed to investigate differences in allele and haplotype frequencies of HLA class II antigens in a sample of Iranian patients with aggressive periodontitis compared with a healthy control group.
Fifty unrelated patients with aggressive periodontitis and 130 healthy volunteers were enrolled in this study. HLA genotyping for HLA-DRB, HLA-DQA1 and HLA-DQB1 was performed using the PCR with sequence-specific primers. Allele and haplotype frequencies were compared across groups.
The frequencies of HLA-DQA103:01, HLA-DQB103:02 and HLA-DQB103:05 alleles, as well as that of the HLA-DRB104:01 allele, were significantly higher in patients with aggressive periodontitis compared with control subjects (p = 0.01, p = 0.04, p = 0.05 and p = 0.04, respectively). In contrast, the frequency of the HLA-DQB10603 allele was significantly lower in patients with aggressive periodontitis compared with control subjects (p = 0.006; odds ratio = 0.20). With regard to haplotype association, a significantly higher frequency of two haplotypes - HLA-DRB104:01/HLA-DQA103:01/HLA-DQB103:02 and HLA-DRB116:01/HLA-DQA101:03/HLA-DQB1*05:01 - was observed in patients with aggressive periodontitis compared with healthy controls (p = 0.01, odds ratio = 2.56 and p = 0.05, odds ratio = 5.38, respectively).
These results provide additional evidence that class II HLA polymorphisms, particularly in the DQ locus, are associated with protection against and susceptibility to aggressive periodontitis.
遗传背景在一系列牙周病的易感性和保护中起着关键作用。与其他传染病一样，人类白细胞抗原（HLA）已被发现与牙周炎有关。本研究旨在调查伊朗侵袭性牙周炎患者样本中 HLA Ⅱ类抗原等位基因和单倍型频率与健康对照组之间的差异。
本研究纳入了 50 名无亲缘关系的侵袭性牙周炎患者和 130 名健康志愿者。采用聚合酶链反应-序列特异性引物法对 HLA-DRB、HLA-DQA1 和 HLA-DQB1 进行基因分型。比较各组间的等位基因和单倍型频率。
与对照组相比，侵袭性牙周炎患者 HLA-DQA103:01、HLA-DQB103:02 和 HLA-DQB103:05 等位基因以及 HLA-DRB104:01 等位基因的频率显著升高（p = 0.01、p = 0.04、p = 0.05 和 p = 0.04）。相反，侵袭性牙周炎患者 HLA-DQB10603 等位基因的频率显著低于对照组（p = 0.006；比值比 = 0.20）。关于单倍型关联，在侵袭性牙周炎患者中，两种单倍型 HLA-DRB104:01/HLA-DQA103:01/HLA-DQB103:02 和 HLA-DRB116:01/HLA-DQA101:03/HLA-DQB1*05:01 的频率明显高于对照组（p = 0.01，比值比 = 2.56 和 p = 0.05，比值比 = 5.38）。
这些结果提供了额外的证据表明，Ⅱ类 HLA 多态性，特别是在 DQ 基因座，与侵袭性牙周炎的保护和易感性有关。
