Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou 310058, PR China.
J Hazard Mater. 2011 Sep 15;192(3):1056-66. doi: 10.1016/j.jhazmat.2011.06.011. Epub 2011 Jul 8.
Greenhouse hydroponic experiments were conducted using Cd-sensitive (cv. Xiushui63) and tolerant (Bing97252) rice genotypes to evaluate how different genotypes responded to Cd toxicity in presence of glutathione (GSH). Results showed that GSH alleviates Cd-toxicity, ameliorates Cd-induced damages on leaf/root ultrastructures. Nine proteins in roots were identified, using 2-DE coupled with mass spectrometry, whose expression were down-regulated in Xiushui63, up-regulated/unchanged in Bing97252 by Cd; coinstantaneously enhanced/unchanged in Cd+GSH over Cd alone treatment in both genotypes. They are l-ascorbate peroxidase, putative short-chain dehydrogenase/reductase, Glycolipid transfer protein, elongation factor, Os04g0652700, carbonic anhydrase, Os08g0374000, chitinase, and putative disease resistance response protein. Eight proteins in leaves with expression of increase in Bing97252 but down-regulate/unchange in Xiushui63, categorized as four groups of their functions: carbon metabolism, TCA cycle, photorespiration and RNA processing. Furthermore, we identified eight proteins with repressed expression in Cd-treated and up-regulated in Cd+GSH-treated rice leaves of Xiushui63.
采用 Cd 敏感型（秀水 63）和耐性型（Bing97252）水稻品种进行温室水培实验，以评估不同基因型在谷胱甘肽（GSH）存在的情况下对 Cd 毒性的反应。结果表明，GSH 缓解了 Cd 毒性，改善了 Cd 对叶片/根超微结构的损伤。利用 2-DE 结合质谱技术，在根中鉴定出 9 种蛋白，其在秀水 63 中的表达下调，在 Bing97252 中 Cd 处理上调/不变；在两种基因型中，Cd+GSH 处理比单独 Cd 处理同时增强/不变。它们是 l-抗坏血酸过氧化物酶、假定的短链脱氢酶/还原酶、糖脂转移蛋白、伸长因子、Os04g0652700、碳酸酐酶、Os08g0374000、几丁质酶和假定的抗病反应蛋白。在 Bing97252 中表达增加而在 Xiushui63 中表达下调/不变的叶片中存在 8 种蛋白，可分为碳代谢、TCA 循环、光呼吸和 RNA 加工 4 组。此外，我们还鉴定出 8 种在 Cd 处理下表达受抑制而在 Xiushui63 的 Cd+GSH 处理下表达上调的叶片蛋白。
