Exogenous Application of Salicylic Acid and Hydrogen Peroxide Ameliorate Cadmium Stress in Milk Thistle by Enhancing Morpho-Physiological Attributes Grown at Two Different Altitudes.

Cadmium (Cd) is a potential and widespread toxic environmental pollutant, mainly derived from a rapid industrial process that has inhibitory effects on growth, physiological, and biochemical attributes of various plant species, including medicinal plants such as L. Gaertn commonly known as milk thistle. Plant signaling molecules, when applied exogenously, help to enhance/activate endogenous biosynthesis of potentially important signaling molecules and antioxidants that boost tolerance against various abiotic stresses, e.g., heavy metal stress. The present study documented the protective role of salicylic acid (SA;0.25 μM) and hydrogen peroxide (HO; 10 μM) priming, foliar spray, and combinational treatments in reducing Cd toxicity (500 μM) in milk thistle grown at two diverse ecological zones of Balochistan Province of Pakistan i.e., Quetta (Qta) and Turbat (Tbt). The morpho-physiological and biochemical attributes of milk thistle were significantly affected by Cd toxicity; however, priming and foliar spray of SA and HO significantly improved the growth attributes (root/shoot length, leaf area, and root/shoot fresh and dry weight), photosynthetic pigments (Chl , , and carotenoids) and secondary metabolites (Anthocyanin, Soluble phenolics, and Tannins) at both altitudes by suppressing the negative impact of Cd. However, the oxidative damage parameters, i.e., MDA and HO, decreased astonishingly under the treatment of signaling molecules, thereby protecting membrane integrity under Cd stress. The morphological variations were profound at the low altitude (Tbt) as compared to the high altitude (Qta). Interestingly, the physiological and biochemical attributes at both altitudes improved under SA and HO treatments, thus hampered the toxic effect of Cd. These signaling compounds enhanced tolerance of plants under heavy metal stress conditions with the consideration of altitudinal, and ambient temperature variations remain to be the key concerns.