Lin Li, Chen Fei, Cai Yue, Chen Zhong-Hua, Cao Fangbin
Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
Fenghua Municipal Bureau of Agriculture and Forestry, Zhejiang, 315500, People's Republic of China.
Environ Sci Pollut Res Int. 2017 Mar;24(8):7009-7015. doi: 10.1007/s11356-017-8399-5. Epub 2017 Jan 14.
Genotypic differences in cadmium (Cd) transport in developing grains of barley (Hordeum vulgare L.) were investigated using detached ears cultured in a nutrient solution containing 0.5 and 5 μM Cd. Cd concentration in each part of the ear in W6nk2 (a low-grain-Cd-accumulation genotype) was much less than in Zhenong8 (a high accumulator) with 0.5 μM Cd treatment. However, Cd concentration in W6nk2 grains increased with an increase in external Cd level and was similar to Zhenong8 with 5 μM Cd treatment. Awn removal, a high relative humidity (RH, 90%) and addition of sucrose markedly decreased grain Cd concentration in Zhenong8 but less affected Cd transport to grain in W6nk2. Stem girdling reduced Cd transport to developing grains with 5 μM Cd treatment, especially for W6nk2, whereas no effect was found in either genotype with low Cd treatment. Our results suggested that higher grain Cd in Zhenong8 is closely related to a larger capacity for xylem transport and is connected with Cd translocation in xylem and phloem sap.
利用在含有0.5和5 μM镉的营养液中培养的离体穗，研究了大麦（Hordeum vulgare L.）发育籽粒中镉（Cd）转运的基因型差异。在0.5 μM镉处理下，W6nk2（低籽粒镉积累基因型）穗各部分的镉浓度远低于浙农8号（高积累型）。然而，W6nk2籽粒中的镉浓度随着外部镉水平的升高而增加，并且在5 μM镉处理下与浙农8号相似。去芒、高相对湿度（RH，90%）和添加蔗糖显著降低了浙农8号籽粒中的镉浓度，但对W6nk2中镉向籽粒的转运影响较小。在5 μM镉处理下，茎环割减少了镉向发育籽粒的转运，尤其是对W6nk2而言，而在低镉处理下，两种基因型均未发现影响。我们的结果表明，浙农8号较高的籽粒镉含量与较大的木质部转运能力密切相关，并且与木质部和韧皮部汁液中的镉转运有关。
