Department of Oral Medical Science, Ohu University School of Dentistry, 31-1 Misumido, Tomitamachi, Koriyama, Fukushima 963 8611, Japan.
Microb Pathog. 2011 Oct;51(4):250-4. doi: 10.1016/j.micpath.2011.06.009. Epub 2011 Jul 1.
Although Candida albicans has been isolated from periodontal pockets, its relationship to periodontitis is unclear. In this study, we investigated the effect of C. albicans on the adhesion and invasion of Ca9-22, a human gingival epithelial cell line, and human gingival fibroblasts by Porphyromonas gingivalis. Heat-killed C. albicans and water-soluble mannoprotein-β-glucan complex from C. albicans (CAWS) did not enhance P. gingivalis adhesion or upregulate the expression of β1 integrin and ICAM-1, which are required for P. gingivalis invasion; both the epithelial cells and fibroblasts expressed dectin-1, which recognizes components of the C. albicans cell wall. However, pretreatment of Ca9-22 cells and human gingival fibroblasts with heat-killed C. albicans or CAWS significantly enhanced P. gingivalis invasion. These results suggest that C. albicans may exacerbate infectious disease by enhancing the invasion of host cells by anaerobic bacteria.
虽然白色念珠菌已从牙周袋中分离出来，但它与牙周炎的关系尚不清楚。在这项研究中，我们研究了白色念珠菌对牙龈卟啉单胞菌对人牙龈上皮细胞系 Ca9-22 和人牙龈成纤维细胞黏附和侵袭的影响。热灭活的白色念珠菌和来自白色念珠菌的水溶性甘露糖蛋白-β-葡聚糖复合物（CAWS）不会增强牙龈卟啉单胞菌的黏附，也不会上调β1 整合素和 ICAM-1 的表达，这些都是牙龈卟啉单胞菌侵袭所必需的；上皮细胞和成纤维细胞都表达了识别白色念珠菌细胞壁成分的 dectin-1。然而，用热灭活的白色念珠菌或 CAWS 预处理 Ca9-22 细胞和人牙龈成纤维细胞可显著增强牙龈卟啉单胞菌的侵袭。这些结果表明，白色念珠菌可能通过增强厌氧菌对宿主细胞的侵袭来加重传染病。
