ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, Faculty of Dentistry, Complutense University, 28040 Madrid, Spain.
Department of Conservative Dentistry, Faculty of Dentistry, University of Chile, Santiago 8380544, Chile.
Int J Mol Sci. 2024 Mar 14;25(6):3277. doi: 10.3390/ijms25063277.
This study aimed to evaluate the impact of on subgingival biofilm formation on dental implant surfaces. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) were used to compare biofilm structure and microbial biomass in the presence and absence of the fungus after periods of 24, 48, and 72 h. Quantitative polymerase chain reaction (qPCR) was used to quantify the number of viable and total micro-organisms for each of the biofilm-forming strains. A general linear model was applied to compare CLSM and qPCR results between the control and test conditions. The biofilm developed with at 72 h had a higher bacterial biomass and a significantly higher cell viability ( < 0.05). After both 48 and 72 h of incubation, in the presence of , there was a significant increase in counts of and and in the cell viability of , , , and . Using a dynamic in vitro multispecies biofilm model, exacerbated the development of the biofilm grown on dental implant surfaces, significantly increasing the number and cell viability of periodontal bacteria.
本研究旨在评估 对牙种植体表面龈下生物膜形成的影响。扫描电子显微镜（SEM）和共聚焦激光扫描显微镜（CLSM）用于比较在有和没有真菌存在的情况下，在 24、48 和 72 小时后生物膜结构和微生物生物量的差异。采用定量聚合酶链反应（qPCR）对每种生物膜形成菌株的活菌数和总微生物数量进行定量。采用一般线性模型比较对照和试验条件下 CLSM 和 qPCR 的结果。在 72 小时时用 培养的生物膜具有更高的细菌生物量和显著更高的细胞活力（<0.05）。在孵育 48 和 72 小时后，在存在 的情况下， 和 的计数以及 、 、 和 的细胞活力均显著增加。使用动态体外多菌种生物膜模型， 加剧了牙种植体表面生物膜的发展，显著增加了牙周细菌的数量和细胞活力。
