Department of Wood Science and Landscape Architecture (BK21 Program), Chonnam National University, Gwangju 500-757, Republic of Korea.
Bioresour Technol. 2011 Sep;102(17):8191-8. doi: 10.1016/j.biortech.2011.06.063. Epub 2011 Jun 23.
Yeast immobilized on alginate beads produced a higher ethanol yield more rapidly than did free yeast cells under the same batch-fermentation conditions. The optimal fermentation conditions were 30°C, pH 5.0, and 10% initial glucose concentration with 2% sodium alginate beads. The fermentation time using reused alginate beads was 10-14 h, whereas fresh beads took 24h, and free cells took 36 h. All bead samples resulted in nearly a 100% ethanol yield, whereas the free cells resulted in an 88% yield. Transmission electron microscopy (TEM) showed that the shortened time and higher yield with the reused beads was due to a higher yeast population per bead as well as a higher porosity. The ultrastructure of calcium alginate beads and the alginate matrix structure known as the "egg-box" model were observed using TEM.
在相同的分批发酵条件下，固定在海藻酸钠珠上的酵母比游离酵母细胞产生更高的乙醇产量，速度更快。最佳发酵条件为 30°C、pH5.0 和 10%初始葡萄糖浓度，使用 2%的海藻酸钠珠。使用重复使用的海藻酸钠珠的发酵时间为 10-14 小时，而新鲜珠则需要 24 小时，游离细胞则需要 36 小时。所有珠样品的乙醇产率几乎达到 100%，而游离细胞的产率为 88%。透射电子显微镜（TEM）显示，重复使用的珠的时间缩短和产率提高是由于每个珠上的酵母种群更高，以及更高的孔隙率。使用 TEM 观察了钙海藻酸钠珠的超微结构和被称为“蛋盒”模型的海藻酸钠基质结构。
