Tianjin Medical University Eye Center, China.
Cancer Biol Ther. 2011 Sep 15;12(6):477-83. doi: 10.4161/cbt.12.6.16300.
Aberrant expression of microRNAs (miRNAs) has been implicated in cancer initiation and progression. In this study, we found that microRNA-34a (miR-34a) is significantly downregulated in glioblastoma multiforme (GBM) specimens compared with normal brain tissues. Growth curve and colony formation assays revealed that miR-34a suppresses proliferation of U373MG and SHG44 glioblastoma cells. Overexpression of miR-34a could induce apoptosis of glioblastoma cells. Also, we identified notch1 as a direct target gene of miR-34a. Knockdown of notch1 showed similar cellular functions as overexpression of miR-34a both in vitro and in vivo. Collectively, our findings show that miR-34a is downregulated in GBM cells and inhibits GBM growth by targeting notch1.
异常表达的 microRNAs（miRNAs）与癌症的发生和发展有关。在这项研究中，我们发现与正常脑组织相比，多形性胶质母细胞瘤（GBM）标本中 microRNA-34a（miR-34a）的表达显著下调。生长曲线和集落形成实验表明，miR-34a 抑制 U373MG 和 SHG44 胶质母细胞瘤细胞的增殖。miR-34a 的过表达可诱导胶质母细胞瘤细胞凋亡。此外，我们还鉴定出 notch1 是 miR-34a 的一个直接靶基因。在体外和体内，notch1 的敲低表现出与 miR-34a 过表达相似的细胞功能。综上所述，我们的研究结果表明，miR-34a 在 GBM 细胞中下调，并通过靶向 notch1 抑制 GBM 的生长。
