Carlson Brett L, Pokorny Jenny L, Schroeder Mark A, Sarkaria Jann N
Mayo Clinic, Department of Radiation Oncology, Rochester, Minnesota, USA.
Curr Protoc Pharmacol. 2011 Mar;Chapter 14(14):Unit 14.16. doi: 10.1002/0471141755.ph1416s52.
Development of clinically relevant tumor model systems for glioblastoma multiforme (GBM) is important for advancement of basic and translational biology. One model that has gained wide acceptance in the neuro-oncology community is the primary xenograft model. This model entails the engraftment of patient tumor specimens into the flank of nude mice and subsequent serial passage of these tumors in the flank of mice. These tumors are then used to establish short-term explant cultures or intracranial xenografts. This unit describes detailed procedures for establishment, maintenance, and utilization of a primary GBM xenograft panel for the purpose of using them as tumor models for basic or translational studies.
开发与临床相关的多形性胶质母细胞瘤（GBM）肿瘤模型系统对于基础生物学和转化生物学的发展至关重要。一种在神经肿瘤学界获得广泛认可的模型是原发性异种移植模型。该模型需要将患者肿瘤标本植入裸鼠侧腹，并随后在小鼠侧腹对这些肿瘤进行连续传代。然后使用这些肿瘤建立短期外植体培养物或颅内异种移植。本单元描述了建立、维持和利用原发性GBM异种移植库的详细程序，目的是将它们用作基础研究或转化研究的肿瘤模型。
