Children's Cancer Institute Australia, Lowy Cancer Research Centre, UNSW Sydney, Sydney, NSW, Australia.
School of Clinical Medicine, Faculty of Medicine & Health, UNSW Sydney, Sydney, NSW, Australia.
Methods Mol Biol. 2024;2806:75-90. doi: 10.1007/978-1-0716-3858-3_7.
The development of clinically relevant and reliable models of central nervous system tumors has been instrumental in advancing the field of Neuro-Oncology. The orthotopic intracranial injection is widely used to study the growth, invasion, and spread of tumors in a controlled environment. Orthotopic models are performed to examine tumor cells isolated from a specific region in a patient in the same site or location in an animal model. Orthotopic brain tumor models are also utilized for preclinical testing of therapeutics as they closely recapitulate the behavior of such cancer and the brain environment of patients. Below, we describe our experiences in the development of murine models of pediatric brain tumors including diffuse midline glioma (DMG), glioblastoma (GBM), and medulloblastoma. The method provides an overview of intracranial stereotactic injections in mice.
临床相关且可靠的中枢神经系统肿瘤模型的发展对于神经肿瘤学领域的发展起到了推动作用。原位颅内注射广泛用于在受控环境中研究肿瘤的生长、侵袭和扩散。原位模型用于检查从患者特定区域分离的肿瘤细胞，在动物模型中在相同部位或位置进行。原位脑肿瘤模型也用于治疗方法的临床前测试，因为它们非常类似于此类癌症的行为和患者的大脑环境。下面，我们描述了我们在开发小儿脑肿瘤模型（包括弥漫性中线胶质瘤（DMG）、胶质母细胞瘤（GBM）和髓母细胞瘤）方面的经验。该方法概述了小鼠颅内立体定向注射。
