一种用于电荷选择性生物聚合物传感的简单策略。

A simple strategy for charge selective biopolymer sensing.

机构信息

Unilever R&D Bangalore, 64 Main Road, Whitefield, Bangalore 560066, India.

出版信息

Chem Commun (Camb). 2011 Aug 21;47(31):8937-9. doi: 10.1039/c1cc12680f. Epub 2011 Jul 8.

Abstract

Polysaccharide-induced protein fluorescence 'turn-on' responses have been studied in the presence of similarly charged micelle at pH ∼3. Quenched protein fluorescence is selectively recovered ('on' state) for bovine serum albumin (BSA) with sodium carboxy methyl cellulose (SCMC) and for pepsin (PS) with chitosan (CS) with starting ultra low concentrations of 0.04 μM and 0.008 μM respectively.

摘要

在 pH 值约为 3 的情况下，研究了多糖诱导的蛋白质荧光“开启”响应在带相同电荷的胶束存在下的情况。用羧甲基纤维素钠（SCMC）使牛血清白蛋白（BSA）和用壳聚糖（CS）使胃蛋白酶（PS）的荧光猝灭得到选择性恢复（“开启”状态），起始超低浓度分别为 0.04 μM 和 0.008 μM。