阳离子化生物聚合物表面用于高蛋白质亲和支撑基质。

Cationically rendered biopolymer surfaces for high protein affinity support matrices.

机构信息

University of Maribor, Institute for the Engineering and Design of Materials, Smetanova Ulica 17, 2000 Maribor, Slovenia.

出版信息

Chem Commun (Camb). 2013 Dec 21;49(98):11530-2. doi: 10.1039/c3cc46414h.

DOI:10.1039/c3cc46414h

PMID:24178232

Abstract

The use of cationic biopolymer surfaces for high protein binding affinity matrices is described. As model proteins, fluorescently labeled bovine serum albumins (FITC-BSA, TRITC-BSA) have been employed. The amount of proteins on such cationically rendered surfaces was quantified by QCM-D. In addition, flexible, transparent, patterned COP slides have been prepared and loaded with proteins ranging from 15 pM to 15 μM TRITC-BSA.

摘要

本文描述了用于高蛋白质结合亲和力基质的阳离子生物聚合物表面的使用。作为模型蛋白，使用了荧光标记的牛血清白蛋白（FITC-BSA、TRITC-BSA）。通过 QCM-D 定量了此类阳离子化表面上的蛋白质含量。此外，还制备了灵活、透明、图案化的 COP 载玻片，并加载了从 15 pM 到 15 μM TRITC-BSA 的蛋白质。