Roholl P J, Elbers H R, Prinsen I, Claessens J A, van Unnik J A
Institute of Pathology, University Hospital Utrecht, The Netherlands.
Hum Pathol. 1990 Dec;21(12):1269-74. doi: 10.1016/s0046-8177(06)80041-9.
The actin immunophenotype of eight benign mesenchymal tumors, 14 nonsarcomatoid tumors, and 46 sarcomatoid tumors was studied, using monoclonal antibodies (MoAb) specific for alpha-smooth muscle actin (clone 1A4), alpha- and gamma-smooth muscle actin (designated CGA7), and muscle actin (designated HHF35) on frozen sections. Tumor cells of nonsarcomatoid tissues were not reactive, but all leiomyomas and five of the seven leiomyosarcomas reacted with the three MoAbs. One leiomyosarcoma was immunoreactive for the MoAb 1A4 only. One of the six malignant schwannomas showed staining for muscle actin (HHF35). The 22 malignant fibrous histocytomas (MFH) expressed these actin isoforms in various degrees. One case immunoreacted with all three MoAbs, three reacted with 1A4 only, seven reacted with CGA7 and HHF35, and two reacted with HHF35 only. Nine MFHs were not immunoreactive for any of the MoAbs specific for (smooth) muscle and actin. In addition, the expression of desmin and collagen type IV was investigated for the group of leiomyosarcomas and MFHs. Desmin was found in five leiomyosarcomas and in two MFHs. Collagen type IV was seen in all leiomyosarcomas, and was seen weakly in a few small areas in four MFHs. When we take into account the expression of all markers tested [( smooth] muscle actin, desmin, and collagen type IV), then six of the 22 MFHs were unreactive for all these markers. Five of these six tumors were located intramuscularly, whereas only half of the total number of MFH cases had an intramuscular location. The fact that 15 of 22 MFHs displayed one or more markers linked with (smooth) muscle differentiation suggests that some of the MFHs may be classified as poorly differentiated leiomyosarcomas, and that MFH is not a unique entity.
利用针对α-平滑肌肌动蛋白（克隆1A4）、α-和γ-平滑肌肌动蛋白（命名为CGA7）以及肌动蛋白（命名为HHF35）的单克隆抗体（MoAb），对8例良性间叶性肿瘤、14例非肉瘤样肿瘤以及46例肉瘤样肿瘤的肌动蛋白免疫表型进行了研究，研究对象为冰冻切片。非肉瘤样组织的肿瘤细胞无反应性，但所有平滑肌瘤以及7例平滑肌肉瘤中的5例与这三种MoAb发生反应。1例平滑肌肉瘤仅对MoAb 1A4呈免疫反应性。6例恶性神经鞘瘤中的1例显示肌动蛋白（HHF35）染色阳性。22例恶性纤维组织细胞瘤（MFH）不同程度地表达这些肌动蛋白异构体。1例病例与所有三种MoAb均发生免疫反应，3例仅与1A4发生反应，7例与CGA7和HHF35发生反应，2例仅与HHF35发生反应。9例MFH对任何针对（平滑）肌和肌动蛋白的MoAb均无免疫反应性。此外，对平滑肌肉瘤组和MFH组还研究了结蛋白和IV型胶原的表达情况。在5例平滑肌肉瘤和2例MFH中发现了结蛋白。IV型胶原在所有平滑肌肉瘤中均可见，在4例MFH的少数小区域中呈弱阳性。当考虑所有检测标志物（[平滑]肌动蛋白、结蛋白和IV型胶原）的表达情况时，22例MFH中有6例对所有这些标志物均无反应性。这6例肿瘤中有5例位于肌肉内，而MFH病例总数中只有一半位于肌肉内。22例MFH中有15例显示一种或多种与（平滑）肌分化相关的标志物，这一事实表明，部分MFH可能可归类为低分化平滑肌肉瘤，且MFH并非一个独特的实体。
