Service de biophysique et médecine nucléaire - Institut de Mécanique des fluides et des Solides CNRS Université de Strasbourg, CHU Hautepierre, Avenue Molière, 67098 Strasbourg Cedex, France.
Pediatr Nephrol. 2012 Jan;27(1):131-8. doi: 10.1007/s00467-011-1945-3. Epub 2011 Jul 9.
This study compares different peritoneal dialysis fluids (PDF) in rats over a short contact time. For greater accuracy, net ultrafiltration (UF) and peritoneal transport indices, mass transfer area coefficient (MTAC) were scaled for the in vivo peritoneal surface area recruited (ivPSA) measured by microcomputerized tomography. Wistar rats underwent nephrectomy (5/6ths), were randomized into two groups and given 1.5% glucose PDF, either conventional acidic lactate (n = 14) or pH neutral bicarbonate (BicaVera) (n = 13); MTAC and UF were measured using a 90-min peritoneal equilibrium test (PET), fill volume (IPV) of 10 ml/100 g; small pore fluid transport was determined from sodium balance and used to calculate free water transport (FWT). Each ivPSA value was significantly correlated with the actual IPV, which varied from one rat to another. At 90 min of contact, there was no difference in recruited ivPSA in relation to PDFs. There was a difference (p < 0.01) in net UF/ivPSA 0.45 vs. 1.41 cm(2)/ml for bicarbonate versus lactate, as there was in the proportion of FWT with bicarbonate (42 ± 5% of net UF) compared to lactate (29 ± 4% of net UF). Net UF for individual values of ivPSA differs between conventional PDF and more biocompatible solutions, such as bicarbonate PDF. This observed change in UF cannot be fully explained by differences in glucose transport. The changes in FWT may be explained by the impact of the PDF biocompatibility on aquaporin function.
本研究比较了短时间接触不同腹膜透析液（PDF）在大鼠中的效果。为了提高准确性，根据微计算机断层扫描测量的体内腹膜表面积（ivPSA），对净超滤（UF）和腹膜转运指数、质量传递面积系数（MTAC）进行了校正。Wistar 大鼠行肾切除术（5/6 肾切除），随机分为两组，分别给予 1.5%葡萄糖 PDF，传统酸性乳酸盐（n = 14）或 pH 中性碳酸氢盐（BicaVera）（n = 13）；使用 90 分钟腹膜平衡试验（PET）测量 MTAC 和 UF，填充体积（IPV）为 10 ml/100 g；从小鼠的钠平衡中确定小孔隙流体转运，并用于计算游离水转运（FWT）。每个 ivPSA 值与实际的 IPV 显著相关，而 IPV 在不同大鼠之间存在差异。在 90 分钟接触时，与 PDFs 相关的募集 ivPSA 没有差异。碳酸氢盐与乳酸盐相比，UF/ivPSA 0.45 与 1.41 cm(2)/ml 之间存在差异（p<0.01），碳酸氢盐的 FWT 比例（UF 的 42±5%）与乳酸盐（UF 的 29±4%）相比也存在差异。对于 ivPSA 的个体值，传统 PDF 和更具生物相容性的溶液（如碳酸氢盐 PDF）之间的 UF 存在差异。这种 UF 的变化不能完全用葡萄糖转运的差异来解释。FWT 的变化可能是由于 PDF 的生物相容性对水通道蛋白功能的影响。
