Klinck Rasmus, Füchtbauer Ernst-Martin, Ahnfelt-Rønne Jonas, Serup Palle, Jensen Jan Nygaard, Jørgensen Mette Christine
Department of Beta Cell Regeneration, Hagedorn Research Institute, Gentofte, Denmark.
Gene Expr Patterns. 2011 Oct;11(7):415-26. doi: 10.1016/j.gep.2011.06.004. Epub 2011 Jul 2.
Expression of the basic helix-loop-helix factor Hairy and Enhancer of Split-1 (Hes1) is required for normal development of a number of tissues during embryonic development. Depending on context, Hes1 may act as a Notch signalling effector which promotes the undifferentiated and proliferative state of progenitor cells, but increasing evidence also points to Notch independent regulation of Hes1 expression. Here we use high resolution confocal scanning of EGFP in a novel BAC transgenic mouse reporter line, Tg(Hes1-EGFP)(1Hri), to analyse Hes1 expression from embryonic day 7.0 (e7.0). Our data recapitulates some previous observations on Hes1 expression and suggests new, hitherto unrecognised expression domains including expression in the definitive endoderm at early somite stages before gut tube closure and thus preceding organogenesis. This mouse line will be a valuable tool for studies addressing the role of Hes1 in a number of different research areas including organ specification, development and regeneration.
在胚胎发育过程中，许多组织的正常发育需要碱性螺旋-环-螺旋因子Hairy和分裂增强子-1（Hes1）的表达。根据具体情况，Hes1可能作为Notch信号效应器，促进祖细胞的未分化和增殖状态，但越来越多的证据也表明Hes1的表达存在Notch非依赖性调节。在这里，我们使用新型BAC转基因小鼠报告系Tg(Hes1-EGFP)(1Hri)对EGFP进行高分辨率共聚焦扫描，以分析从胚胎第7.0天（e7.0）开始的Hes1表达。我们的数据重现了一些先前关于Hes1表达的观察结果，并提示了新的、迄今未被认识的表达域，包括在肠管闭合之前、 somitogenesis早期的定形内胚层中的表达，因此早于器官发生。该小鼠品系将成为研究Hes1在包括器官特化、发育和再生在内的许多不同研究领域中的作用的有价值工具。
