烟曲霉和白假丝酵母菌对囊性纤维化上皮细胞促炎反应的影响。
Effect of Aspergillus fumigatus and Candida albicans on pro-inflammatory response in cystic fibrosis epithelium.
机构信息
Respiratory Medicine Research Group, Centre for Infection and Immunity, Queens University Belfast, Health Sciences Building, 97 Lisburn Road, Belfast, Northern Ireland, BT9 7BL, UK.
出版信息
J Cyst Fibros. 2011 Dec;10(6):401-6. doi: 10.1016/j.jcf.2011.06.006. Epub 2011 Jul 13.
BACKGROUND
The identification of filamentous fungi and/or yeasts in the airway secretions of individuals with cystic fibrosis (CF) is becoming increasingly prevalent; yet the importance of these organisms in relation to underlying inflammation is poorly defined.
METHODS
Cystic fibrosis bronchial epithelial cells (CFBE) and human bronchial epithelial cells (HBE) were co-incubated with Candida albicans whole cells or Aspergillus fumigatus conidia for 24 h prior to the measurement of pro-inflammatory cytokines IL-6 and IL-8 by ELISA.
RESULTS
Treatment of HBE or CFBE with C. albicans whole cells did not alter cytokine secretion. However treatment of CFBE with A. fumigatus conidia resulted in a 1.45-fold increase in IL-6 and a 1.65-fold increase in IL-8 secretion in comparison to basal levels; in contrast there was far less secretion from HBE cells.
CONCLUSION
Our data indicate that A. fumigatus infection modulates a pro-inflammatory response in CF epithelial cells while C. albicans does not.
背景
在囊性纤维化（CF）患者的气道分泌物中鉴定丝状真菌和/或酵母菌越来越普遍；然而，这些生物体与潜在炎症的关系尚未明确。
方法
在测量 ELISA 法测定前，将囊性纤维化支气管上皮细胞（CFBE）和人支气管上皮细胞（HBE）与白色念珠菌全细胞或烟曲霉分生孢子共同孵育 24 小时。
结果
用白色念珠菌全细胞处理 HBE 或 CFBE 不会改变细胞因子的分泌。然而，与基础水平相比，用烟曲霉分生孢子处理 CFBE 导致 IL-6 分泌增加 1.45 倍，IL-8 分泌增加 1.65 倍；相比之下，HBE 细胞的分泌量要少得多。
结论
我们的数据表明，与白色念珠菌不同，烟曲霉感染会调节 CF 上皮细胞的促炎反应。
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