Division of Cardiovascular Medicine, Kanazawa University Graduate School of Medicine, Japan.
Circ J. 2011;75(9):2260-8. doi: 10.1253/circj.cj-11-0246. Epub 2011 Jul 12.
Mesenchymal stem cells (MSC) are multipotent and reside in bone marrow (BM), adipose tissue and many other tissues. However, the molecular foundations underlying the differences in proliferation, differentiation potential and paracrine effects between adipose tissue-derived MSC (ASC) and BM-derived MSC (BM-MSC) are not well-known. Therefore, we investigated differences in the gene and secretory protein expressions of the 2 types of MSC.
ASC and BM-MSC were obtained from subcutaneous adipose tissue and BM of adult Lewis rats. ASC proliferated as rapidly as BM-MSC, and had expanded 200-fold in approximately 2 weeks. On microarray analysis of 31,099 genes, 571 (1.8%) were more highly (>3-fold) expressed in ASC, and a number of these genes were associated with mitosis and immune response. On the other hand, 571 genes (1.8%) were more highly expressed in BM-MSC, and some of these genes were associated with organ development and morphogenesis. In secretory protein analysis, ASC secreted significantly larger amounts of growth factor and inflammatory cytokines, such as vascular endothelial growth factor, hepatocyte growth factor and interleukin 6, whereas BM-MSC secreted significantly larger amounts of stromal-derived factor-1α.
There are significant differences between ASC and BM-MSC in the cytokine secretome, which may provide clues to the molecule mechanisms associated with tissue regeneration and alternative cell sources.
间充质干细胞（MSC）具有多能性，存在于骨髓（BM）、脂肪组织和许多其他组织中。然而，脂肪组织来源的间充质干细胞（ASC）和 BM 来源的间充质干细胞（BM-MSC）在增殖、分化潜能和旁分泌效应方面的差异的分子基础尚不清楚。因此，我们研究了这两种 MSC 之间的基因和分泌蛋白表达的差异。
从成年 Lewis 大鼠的皮下脂肪组织和 BM 中获得 ASC 和 BM-MSC。ASC 的增殖速度与 BM-MSC 一样快，大约 2 周内就扩增了 200 倍。在对 31099 个基因的微阵列分析中，571 个（1.8%）在 ASC 中表达更高（>3 倍），其中一些基因与有丝分裂和免疫反应有关。另一方面，571 个基因（1.8%）在 BM-MSC 中表达更高，其中一些基因与器官发育和形态发生有关。在分泌蛋白分析中，ASC 分泌的生长因子和炎症细胞因子（如血管内皮生长因子、肝细胞生长因子和白细胞介素 6）的量明显更大，而 BM-MSC 分泌的基质衍生因子-1α的量明显更大。
ASC 和 BM-MSC 在细胞因子分泌组中有显著差异，这可能为与组织再生和替代细胞来源相关的分子机制提供线索。
