Institute of Farm Animal Genetics and Reproduction, NPPC, Research Institute for Animal Production in Nitra, Hlohovecká 2, 951 41 Lužianky, Slovakia.
Institute of Biotechnology, Faculty of Biotechnology and Food Science, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 949 76 Nitra, Slovakia.
Int J Mol Sci. 2021 Nov 13;22(22):12283. doi: 10.3390/ijms222212283.
Human adipose tissue-derived mesenchymal stem cells (AT-MSCs) have been studied several years for their immunomodulatory effect through the paracrine mechanism and cytokine secretion. In combination with endothelial progenitor cells (EPCs), MSCs have great therapeutical potential for the repair of endothelium and wound healing. However, little is known about the cytokine profile of rabbit AT-MSCs or even EPCs. The aim of this study was to analyze the secretomes of these rabbit stem/progenitor cells. A large-scale human cytokine array (up to 80 cytokines) was used to identify and compare cytokines secreted into conditioned media of human and rabbit AT-MSCs as well as HUVECs and rabbit EPCs. Few cytokines were highly expressed by human AT-MSCs (TIMP-2, TIMP-1), HUVECs (MCP-1, TIMP-2, GRO, Angiogenin, IL-8, TIMP-1), or by rabbit EPCs (TIMP-2). Several cytokines have moderate expression by human (MCP-1, GRO, Angiogenin, TGF-β 2, IL-8, LIF, IL-6, Osteopontin, Osteoprotegerin) and rabbit AT-MSCs (TIMP-2, TGF-β 2, LIF, Osteopontin, IL-8, IL-5, IL-3) or by HUVECs (IL-6, MIF, TGF-β 2, GCP-2, IGFBP-2, Osteoprotegerin, EGF, LIF, PDGF-BB, MCP-3, Osteopontin, Leptin, IL-5, ENA-78, TNF-β) and rabbit EPCs (TGF-β 2, Osteopontin, GRO, LIF, IL-8, IL-5, IL-3). In conclusion, the proposed method seems to be useful for the secretome analysis of rabbit stem/progenitor cells.
人脂肪组织来源的间充质干细胞（AT-MSCs）已通过旁分泌机制和细胞因子分泌研究了多年，具有免疫调节作用。与内皮祖细胞（EPCs）结合使用时，MSCs 在修复内皮和伤口愈合方面具有巨大的治疗潜力。然而，对于兔 AT-MSCs 甚至 EPCs 的细胞因子谱知之甚少。本研究旨在分析这些兔干细胞/祖细胞的分泌组。使用大型人类细胞因子阵列（多达 80 种细胞因子）来鉴定和比较人源和兔源 AT-MSCs 以及 HUVECs 和兔 EPCs 分泌到条件培养基中的细胞因子。人类 AT-MSCs（TIMP-2、TIMP-1）、HUVECs（MCP-1、TIMP-2、GRO、血管生成素、IL-8、TIMP-1）或兔 EPCs（TIMP-2）高表达少数细胞因子。几种细胞因子在人类（MCP-1、GRO、血管生成素、TGF-β2、IL-8、LIF、IL-6、骨桥蛋白、骨保护素）和兔 AT-MSCs（TIMP-2、TGF-β2、LIF、骨桥蛋白、IL-8、IL-5、IL-3）或 HUVECs（IL-6、MIF、TGF-β2、GCP-2、IGFBP-2、骨保护素、EGF、LIF、PDGF-BB、MCP-3、骨桥蛋白、瘦素、IL-5、ENA-78、TNF-β）和兔 EPCs（TGF-β2、骨桥蛋白、GRO、LIF、IL-8、IL-5、IL-3）中呈中度表达。总之，该方法似乎可用于兔干细胞/祖细胞的分泌组分析。
