β-肾上腺素能受体通过环磷酸腺苷对大鼠皮质和小脑星形胶质细胞中神经生长因子表达的调节。
Beta-adrenergic receptor regulation, through cyclic AMP, of nerve growth factor expression in rat cortical and cerebellar astrocytes.
作者信息
Schwartz J P, Mishler K
机构信息
Clinical Neuroscience Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892.
出版信息
Cell Mol Neurobiol. 1990 Sep;10(3):447-57. doi: 10.1007/BF00711186.
Abstract
- Type 1 astrocytes prepared from 3-day rat cortex and cerebellum express the 1.3-kb nerve growth factor (NGF) mRNA and synthesize and release beta-NGF. 2. Isoproterenol (IP), a beta-adrenergic agonist, stimulates NGF mRNA content in cortical astrocytes; this increase is blocked by the beta-adrenergic antagonist propranolol but not the alpha-antagonist phenoxybenzamine. The EC50 for the effect of IP is 5 nM. 3. IP increases astrocyte cyclic AMP as does forskolin, which directly activates adenylate cyclase and also increases NGF mRNA content. Cerebellar astrocytes contain about one-third as much NGF mRNA, which can also be increased by forskolin and cyclic AMP. 4. These results suggest that CNS astrocytes can serve as a source of NGF and that the NGF gene is one of the class of cyclic AMP regulated genes.
摘要
- 从3日龄大鼠皮层和小脑中制备的1型星形胶质细胞表达1.3 kb的神经生长因子（NGF）mRNA，并合成和释放β-NGF。2. 异丙肾上腺素（IP），一种β-肾上腺素能激动剂，刺激皮层星形胶质细胞中NGF mRNA的含量；这种增加被β-肾上腺素能拮抗剂普萘洛尔阻断，但不被α-拮抗剂酚苄明阻断。IP作用的EC50为5 nM。3. IP增加星形胶质细胞环磷酸腺苷（cAMP），福斯可林也有此作用，福斯可林直接激活腺苷酸环化酶，也增加NGF mRNA含量。小脑星形胶质细胞含有的NGF mRNA约为其三分之一，福斯可林和环磷酸腺苷也可增加其含量。4. 这些结果表明中枢神经系统星形胶质细胞可作为NGF的来源，且NGF基因是环磷酸腺苷调节基因类中的一种。
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