Sato Kazuomi, Toriyama Masaru
Department of Life Science, College of Agriculture, Tamagawa University, 6-1-1 Tamagawa-Gakuen, Tokyo, Japan.
Int J Mol Sci. 2011;12(6):3998-4008. doi: 10.3390/ijms12063998. Epub 2011 Jun 14.
In the present work, we investigated the effect of non-steroidal anti-inflammatory drugs (NSAIDs) on the monophenolase and diphenolase activity of mushroom tyrosinase. The results showed that diflunisal and indomethacin inhibited both monophenolase and diphenolase activity. For monophenolase activity, the lag time was extended in the presence of diflunisal. In the presence of indomethacin, the lag time did not change. IC(50) values of monophenolase activity were estimated to be 0.112 mM (diflunisal) and 1.78 mM (indomethacin). Kinetic studies of monophenolase activity revealed that both diflunisal and indomethacin were non-competitive inhibitors. For diphenolase activity, IC(50) values were estimated to be 0.197 mM (diflunisal) and 0.509 mM (indomethacin). Diflunisal and indomethacin were also found to be non-competitive diphenolase inhibitors.
在本研究中，我们考察了非甾体抗炎药（NSAIDs）对蘑菇酪氨酸酶单酚酶和二酚酶活性的影响。结果表明，双氯芬酸和吲哚美辛均抑制单酚酶和二酚酶活性。对于单酚酶活性，在双氯芬酸存在下延迟时间延长。在吲哚美辛存在下，延迟时间未改变。单酚酶活性的IC(50)值估计为0.112 mM（双氯芬酸）和1.78 mM（吲哚美辛）。单酚酶活性的动力学研究表明，双氯芬酸和吲哚美辛均为非竞争性抑制剂。对于二酚酶活性，IC(50)值估计为0.197 mM（双氯芬酸）和0.509 mM（吲哚美辛）。双氯芬酸和吲哚美辛也被发现为非竞争性二酚酶抑制剂。
