Shi Xiao-li, Ren Yue-zhong, Wu Jing
Department of Endocrinology, Second Affiliated Hospital of Medical College, Zhejiang University, Hangzhou 310009, China.
Exp Diabetes Res. 2011;2011:754673. doi: 10.1155/2011/754673. Epub 2011 Jun 21.
To investigate the effect of intermittent high glucose (IHG) and sustained high glucose (SHG) on inducing β-cell apoptosis and the potential involved mechanisms, INS-1 beta cells were incubated for 72 h in the medium containing different glucose concentrations: control (5.5 mmol/L), SHG (33.3 mmol/L), and IHG (5.5 mmol/L and 33.3 mmol/L glucose alternating every 12 h). Cell viability, apoptosis rate, and oxidative-stress markers were determined. The results showed that the apoptosis induced by IHG was more obvious than that by SHG. Simultaneously, the intracellular level of oxidative stress was more significantly increased in INS-1 cells exposed to IHG. These findings suggest that intermittent high glucose could be more deleterious to β-cell than a constant high concentration of glucose, this may be due to the aggravation of oxidative stress triggered by intermittent high glucose.
为研究间歇性高糖（IHG）和持续性高糖（SHG）对诱导β细胞凋亡的影响及其潜在机制，将INS-1β细胞在含有不同葡萄糖浓度的培养基中培养72小时：对照组（5.5 mmol/L）、SHG组（33.3 mmol/L）和IHG组（5.5 mmol/L和33.3 mmol/L葡萄糖每12小时交替一次）。测定细胞活力、凋亡率和氧化应激标志物。结果显示，IHG诱导的凋亡比SHG更明显。同时，暴露于IHG的INS-1细胞内氧化应激水平显著升高。这些发现表明，间歇性高糖可能比持续高浓度葡萄糖对β细胞更具危害性，这可能是由于间歇性高糖引发的氧化应激加剧所致。
