Haviarová Zora, Janegová Andrea, Janega Pavel, Durdík Stefan, Kováč Peter, Stvrtinová Viera, Mráz Peter
Department of Anatomy, Faculty of Medicine, Comenius University, Sasinkova 2-4, 81372 Bratislava, Slovakia.
Int J Vasc Med. 2011;2011:204723. doi: 10.1155/2011/204723. Epub 2011 May 29.
There are conflicting findings in literature about the structural changes of the primary varicose veins. NO (a potent vasodilatator) is synthesized by nitric oxide synthase (NOS). From 3 known NOS isoforms the two are constitutional: eNOS (endothelial NOS) and nNOS (neuronal NOS). 10 varicose and 10 control vein samples were processed by standard light microscopy and immuno-histochemica techniques using rabbit polyclonal antibodies against eNOS and nNOS. Antibodies expression was evaluated semiquantitatively and proved morphometrically by 2D-image analysis. total area of NOS isoforms expressions was determined by color analysis and color digital subtraction. The results showed discontinuous and significantly lower expression of both NOS isoforms the in the tunica media of varicose veins compared with the control group. For the statistical analysis the unpaired t-test was used. Our results suppose lower NO levels in varicose vein wall, deducing that varicose dilatation is due to other mechanism, and they contradict the results of previously published similar works.
关于原发性静脉曲张的结构变化，文献中的研究结果相互矛盾。一氧化氮（一种强效血管扩张剂）由一氧化氮合酶（NOS）合成。在已知的三种NOS同工型中，有两种是组成型的：内皮型一氧化氮合酶（eNOS）和神经元型一氧化氮合酶（nNOS）。使用抗eNOS和nNOS的兔多克隆抗体，通过标准光学显微镜和免疫组织化学技术对10个静脉曲张静脉样本和10个对照静脉样本进行处理。抗体表达通过半定量评估，并通过二维图像分析进行形态计量学验证。通过颜色分析和彩色数字减法确定NOS同工型表达的总面积。结果显示，与对照组相比，静脉曲张静脉中膜中两种NOS同工型的表达均不连续且显著降低。统计分析采用非配对t检验。我们的结果推测静脉曲张静脉壁中的一氧化氮水平较低，推断静脉曲张扩张是由其他机制引起的，这与之前发表的类似研究结果相矛盾。
