Esteban F J, Jiménez A, Barroso J B, Pedrosa J A, del Moral M L, Rodrigo J, Peinado M A
Department of Experimental Biology, Faculty of Sciences, University of Jaén, Spain.
J Anat. 1998 Aug;193 ( Pt 2)(Pt 2):241-9. doi: 10.1046/j.1469-7580.1998.19320241.x.
We have explored the innervation of the rainbow trout (O. mykiss) liver using immunohistochemical procedures and light microscopy to detect in situ protein gene product 9.5 and neuronal nitric oxide synthase immunoreactivities (PGP-IR and NOS-IR). The results showed PGP-IR nerve fibres running with the extralobular biliary duct (EBD), hepatic artery (EHA) and portal vein (EPV) that form the hepatic hilum, as well as following the spatial distribution of the intrahepatic blood vessel and biliary channels. These nerve fibres appear as single varicose processes, thin bundles, or thick bundles depending on their diameter and location in the wall of the blood vessel or biliary duct. No PGP-IR fibres were detected in the liver parenchyma. NOS-IR nerve fibres were located only in the vessels and ducts that form the hepatic hilum (EBD, EHA, EPV); in addition, NOS-IR nerve cell bodies were found isolated or forming ganglionated plexuses in the peribiliary fibromuscular tissue of the EBD. No PGP-IR ganglionated plexuses were detected in the EBD. The location of the general (PGP-IR) and nitrergic (nNOS-IR) intrinsic nerves of the trout liver suggest a conserved evolutionary role of the nervous control of hepatic blood flow and hepatobiliary activity.
我们采用免疫组织化学方法和光学显微镜，通过检测原位蛋白基因产物9.5和神经元型一氧化氮合酶免疫反应性（PGP-IR和NOS-IR），探究了虹鳟（O. mykiss）肝脏的神经支配情况。结果显示，PGP-IR神经纤维与构成肝门的小叶外胆管（EBD）、肝动脉（EHA）和门静脉（EPV）伴行，并沿着肝内血管和胆管的空间分布走行。这些神经纤维根据其直径以及在血管或胆管壁中的位置，呈现为单个曲张的突起、细束或粗束。在肝实质中未检测到PGP-IR纤维。NOS-IR神经纤维仅位于构成肝门的血管和胆管（EBD, EHA, EPV）中；此外，在EBD的胆管周围纤维肌肉组织中发现了孤立的或形成神经节丛的NOS-IR神经细胞体。在EBD中未检测到PGP-IR神经节丛。虹鳟肝脏中一般（PGP-IR）和含氮能（nNOS-IR）的内在神经的位置表明，神经对肝血流和肝胆活动的控制在进化过程中具有保守作用。
