Elks Philip M, Loynes Catherine A, Renshaw Stephen A
MRC Centre for Developmental and Biomedical Genetics, The University of Sheffield, Sheffield, UK.
Methods Mol Biol. 2011;769:261-75. doi: 10.1007/978-1-61779-207-6_18.
A key feature of inflammatory cells is the ability to migrate to a site of injury or infection quickly and efficiently. Infectious agents can then be taken up by these inflammatory cells, preventing established infection. Inflammatory cell migration is driven by a complex interaction between inflammatory cells and their environment. In order to maintain health, inflammation needs to resolve, allowing the surrounding tissues to recover and heal. These processes are not fully understood and have been difficult to study in cell culture due to the complex interactions between cell types. We therefore use a range of techniques in near-transparent zebrafish (Danio rerio) larvae to study these migration events in a whole-organism, in vivo model. Using a transgenic zebrafish line that specifically marks neutrophils with green fluorescent protein, Tg(mpx:GFP)i114, we are able to follow neutrophil behaviour at a single cell level. Using these methods, the cellular processes involved in all phases of inflammation can be studied and better understood.
炎症细胞的一个关键特征是能够迅速且高效地迁移到损伤或感染部位。然后，这些炎症细胞可以摄取感染因子，防止感染的形成。炎症细胞的迁移是由炎症细胞与其环境之间复杂的相互作用驱动的。为了维持健康，炎症需要消退，使周围组织得以恢复和愈合。由于细胞类型之间复杂的相互作用，这些过程尚未完全被理解，并且在细胞培养中很难进行研究。因此，我们使用一系列技术，在近乎透明的斑马鱼（Danio rerio）幼体中，在全生物体的体内模型中研究这些迁移事件。使用一种转基因斑马鱼品系Tg(mpx:GFP)i114，该品系用绿色荧光蛋白特异性标记中性粒细胞，我们能够在单细胞水平上追踪中性粒细胞的行为。使用这些方法，可以研究和更好地理解炎症各个阶段所涉及的细胞过程。
