Łukaszewicz-Hussain Anna
Uniwersytet Medyczny, Białystok Załdad Toksykologii.
Med Pr. 2011;62(1):23-9.
Toxicity of organophosphate insecticides is mainly due to the inhibition of acetylcholinesterase (AChE). However organophosphate insecticides in acute as well as in chronic and subchronic intoxication may lead to oxidative stress causing enhancement of lipid peroxidation and changing the activities of antioxidative enzymes and concentration of non-enzymatic antioxidant. For this reason the aim of the work was to estimate glutathione and hydrogen peroxide levels in the liver, as well as the concentration of total glutathione in serum of rats in subchronic intoxication with chlorfenvinphos.
The animals received chlorfenvinphos, intragastrically with use of a stomach tube, at a one daily dose of 0.3 mg/kg/day for 14 or 28 days. For biochemical determinations BIOXYTECH GSH-400 and BIOXYTECH H2O2-560 Assay kit, OXIS International, Inc., Portland, U.S.A. (reduced glutathione and hydrogen peroxide), and Glutathione Assay Kit, Cayman Chemical Company, U.S.A. (determination of serum total glutathione level) were used.
Chlorfenvinphos administration resulted in a decreased level of reduced glutathione in liver accompanied by an increase in liver hydrogen peroxide and serum total glutathione concentrations. The observed changes were more pronounced after 28 days of intoxication.
The common use of organophosphate insecticides results in the environmental pollution, therefore, the decreased liver glutathione level is an additional risk factor for people treated with different medicine (e.g., paracetamol).
有机磷杀虫剂的毒性主要归因于乙酰胆碱酯酶（AChE）的抑制作用。然而，有机磷杀虫剂在急性、慢性和亚慢性中毒时均可能导致氧化应激，引起脂质过氧化增强，改变抗氧化酶的活性和非酶抗氧化剂的浓度。因此，本研究的目的是评估用毒虫畏进行亚慢性中毒的大鼠肝脏中的谷胱甘肽和过氧化氢水平，以及血清中总谷胱甘肽的浓度。
动物通过胃管经口给予毒虫畏，每日剂量为0.3 mg/kg/天，持续14天或28天。生化测定使用美国波特兰OXIS国际公司的BIOXYTECH GSH - 400和BIOXYTECH H2O2 - 560检测试剂盒（用于检测还原型谷胱甘肽和过氧化氢），以及美国开曼化学公司的谷胱甘肽检测试剂盒（用于测定血清总谷胱甘肽水平）。
给予毒虫畏导致肝脏中还原型谷胱甘肽水平降低，同时肝脏过氧化氢和血清总谷胱甘肽浓度升高。中毒28天后观察到的变化更为明显。
有机磷杀虫剂的广泛使用导致环境污染，因此，肝脏谷胱甘肽水平降低是接受不同药物（如对乙酰氨基酚）治疗的人群的一个额外风险因素。
