Département de phytologie, Université Laval, Québec, Québec, Canada.
Mol Ecol Resour. 2011 Nov;11(6):1022-32. doi: 10.1111/j.1755-0998.2011.03047.x. Epub 2011 Jul 12.
Intraguild predation (IGP) has been increasingly recognized as an important interaction in ecological systems over the past two decades, and remarkable insights have been gained into its nature and prevalence. We have developed a technique using molecular gut-content analysis to compare the rate of IGP between closely related species of coccinellid beetles (lady beetles or ladybirds), which had been previously known to prey upon one another. We first developed PCR primers for each of four lady beetle species: Harmonia axyridis, Coccinella septempunctata, Coleomegilla maculata and Propylea quatuordecimpunctata. We next determined the prey DNA detection success over time (DS(50) ) for each combination of interacting species following a meal. We found that DS(50) values varied greatly between predator-prey combinations, ranging from 5.2 to 19.3 h. As a result, general patterns of detection times based upon predator or prey species alone are not discernable. We used the DS(50) values to correct field data to demonstrate the importance of compensation for detection times that are specific to particular predator-prey combinations.
在过去的二十年中，种内捕食（IGP）已被越来越多地认为是生态系统中的一种重要相互作用，并且对其性质和普遍性有了显著的了解。我们开发了一种使用分子肠道内容物分析的技术，来比较先前已知相互捕食的近缘瓢虫（瓢虫或瓢虫）物种之间的 IGP 率。我们首先为四种瓢虫物种：Harmonia axyridis、Coccinella septempunctata、Coleomegilla maculata 和 Propylea quatuordecimpunctata 各自开发了 PCR 引物。接下来，我们确定了每种相互作用物种组合在进食后随时间推移的猎物 DNA 检测成功率（DS(50)）。我们发现，DS(50) 值在捕食者-猎物组合之间差异很大，范围从 5.2 到 19.3 小时。因此，仅基于捕食者或猎物物种的检测时间的一般模式是不可辨别。我们使用 DS(50) 值来校正现场数据，以证明对特定捕食者-猎物组合的检测时间进行补偿的重要性。
