Department of Infectious Diseases, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
J Biomater Appl. 2012 Sep;27(3):359-66. doi: 10.1177/0885328211406299. Epub 2011 Jul 12.
In this study, we evaluated a porous and single-layer skin polyethersulfone (PES) membrane as a material for use in hybrid bioartificial liver support systems. The PES membrane has been characterized as a single-layer skin structure, with a rough porous surface. Specifically, we studied the ability of the human hepatoblastoma cell lines (HepG2) to adhere, grow, and spread on the PES membrane. Furthermore, we examined albumin secretion, low-density lipoprotein uptake, and CYP450 activity of HepG2 cells that grew on the membrane. HepG2 cells readily adhered onto the outer surfaces of PES membranes. Over time, HepG2 cells proliferated actively, and confluent monolayer of cells covered the available surface area of the membrane, eventually forming cell clusters and three-dimensional aggregates. Furthermore, HepG2 cells grown on PES membranes maintained highly specific functions, including uptake capability, biosynthesis and biotransformation. These results indicate that PES membranes are potential substrates for the growth of human liver cells and may be useful in the construction of hollow fiber bioreactors. Porous and single-layer skin PES membranes and HepG2 cells may be potential biomaterials for the development of biohybrid liver devices.
在这项研究中，我们评估了多孔单层聚醚砜（PES）膜作为用于混合生物人工肝支持系统的材料。PES 膜被描述为具有粗糙多孔表面的单层皮肤结构。具体而言，我们研究了人肝癌细胞系（HepG2）在 PES 膜上附着、生长和扩散的能力。此外，我们研究了在膜上生长的 HepG2 细胞的白蛋白分泌、低密度脂蛋白摄取和 CYP450 活性。HepG2 细胞容易附着在 PES 膜的外表面。随着时间的推移，HepG2 细胞积极增殖，细胞单层铺满了膜的可用表面积，最终形成细胞簇和三维聚集体。此外，在 PES 膜上生长的 HepG2 细胞保持了高度特异性的功能，包括摄取能力、生物合成和生物转化。这些结果表明，PES 膜是人类肝细胞生长的潜在基质，可能有助于构建中空纤维生物反应器。多孔单层皮肤 PES 膜和 HepG2 细胞可能是开发生物杂交肝装置的潜在生物材料。
