免疫荧光技术在猪传染性胃肠炎实验室诊断中的应用。
The use of immunofluorescence techniques for the laboratory diagnosis of transmissible gastroenteritis of swine.
作者信息
Solorzano R F, Morin M, Morehouse L G
出版信息
Can J Comp Med. 1978 Oct;42(4):385-91.
Over a four year period, 74 of 250 field outbreaks of enteric disease (30%) and 110 of 440 swine (25%) were positive for transmissible gastroenteritis by immunofluorescence procedures. Of 141 swine from herds positive for transmissible gastroenteritis 110 (78%) were positive by fluorescent antibody techniques. The fastest, easiest to perform and most effective procedure was the examination of frozen sections of the jejunum from acutely ill animals by the fluorescent antibody tissue section technique. Only two herds were found to be positive by the fluorescent antibody tissue culture technique which were negative by fluorescent antibody tissue section technique. A considerable number of outbreaks, 21 of 74 (28%), of transmissible gastroenteritis were detected by immunofluorescence in swine over two weeks of age. The majority of outbreaks of transmissible gastroenteritis, 50 of 74 (68%), occurred in Missouri during the months of January through April and 63 of 74 (85%) during the months of December through May. The recurrence of the disease in a number of counties over a four-year period suggest the possibility of endemic foci.
在四年期间，通过免疫荧光程序检测，250起肠道疾病现场暴发中有74起（30%）呈传染性胃肠炎阳性，440头猪中有110头（25%）呈阳性。在传染性胃肠炎呈阳性的猪群中，141头猪中有110头（78%）通过荧光抗体技术检测呈阳性。最快、最易操作且最有效的方法是采用荧光抗体组织切片技术检查急性病猪空肠的冷冻切片。只有两个猪群通过荧光抗体组织培养技术检测呈阳性，但通过荧光抗体组织切片技术检测呈阴性。在两周龄以上的猪中，通过免疫荧光检测出相当数量（74起中的21起，占28%）的传染性胃肠炎暴发。大多数传染性胃肠炎暴发（74起中的50起，占68%）发生在密苏里州的1月至4月，74起中的63起（85%）发生在12月至次年5月。在四年期间，多个县出现该疾病的复发，这表明存在地方性疫源地的可能性。
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