Janke B H, Morehouse L G, Solorzano R F
Department of Veterinary Pathology, College of Veterinary Medicine, University of Missouri, Columbia 65211.
Can J Vet Res. 1988 Jul;52(3):360-3.
Three rotaviruses and three enteroviruses were isolated from pigs with diarrhea. The three enteroviruses and one of the rotaviruses were recovered from pigs infected with both viruses. Separation of rotaviruses and enteroviruses from tissues containing both viruses was effected by pancreatin treatment, terminal dilution, and inoculation onto different cell lines. The three rotaviruses were group A serotype 1, and the enteroviruses were serotypes 2, 3 and 7. Cell culture preparations of these six viruses were inoculated into colostrum-deprived neonatal pigs. All of the rotavirus and enterovirus isolates established intestinal and systemic infection and were shed in the feces after oral inoculation. Concurrent infection with both viruses resulted in only minor alteration of systemic distribution and did not alter fecal shedding of either virus.
从腹泻猪中分离出三种轮状病毒和三种肠道病毒。三种肠道病毒和其中一种轮状病毒是从同时感染这两种病毒的猪中分离出来的。通过胰蛋白酶处理、终点稀释以及接种到不同细胞系，从含有这两种病毒的组织中分离出轮状病毒和肠道病毒。三种轮状病毒为A组1型，肠道病毒为2型、3型和7型。将这六种病毒的细胞培养物接种到初乳缺乏的新生仔猪体内。所有分离出的轮状病毒和肠道病毒均引发肠道和全身感染，并在口服接种后随粪便排出。两种病毒同时感染仅导致全身分布略有改变，且不影响任何一种病毒的粪便排出。
