Gurov A V, Favorov M O, Iashina T L, Gol'dberg E Z, Khizhniakova T M, Anan'ev V A
Vopr Virusol. 1990 Jul-Aug;35(4):306-8.
In transformation by Epstein-Barr virus of lymphocytes derived from patients with acute hepatitis B, continuous cell cultures were obtained which produced anti-HBc IgM antibodies. These cell lines underwent from 50 to 150 passages. The level of the specific immunoglobulin production was shown to have a trend to decline; however, after cloning the antibody production became more stable. The antibodies produced by the cloned lymphocyte culture could not neutralize the antigen (unlike polyclonal antibodies) which indicated a high efficacy of cloning. When the solid phase was sensitized with produced immunoglobulins, specific activity of binding of HBc antigen detectable by anti-HBc conjugate with horseradish peroxidase was demonstrated. The study of sedimentation properties of antibodies produced by transformed lymphocytes showed their sedimentation constant to be 19S.
在对急性乙型肝炎患者来源的淋巴细胞进行爱泼斯坦-巴尔病毒转化时，获得了能产生抗-HBc IgM抗体的连续细胞培养物。这些细胞系传代了50至150次。特异性免疫球蛋白的产生水平呈下降趋势；然而，克隆后抗体产生变得更加稳定。克隆的淋巴细胞培养物产生的抗体不能中和抗原（与多克隆抗体不同），这表明克隆效率很高。当用产生的免疫球蛋白致敏固相时，证明了用辣根过氧化物酶抗-HBc缀合物可检测到的HBc抗原结合的特异性活性。对转化淋巴细胞产生的抗体的沉降特性研究表明其沉降常数为19S。
