Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Angew Chem Int Ed Engl. 2011 Aug 22;50(35):8077-81. doi: 10.1002/anie.201102646. Epub 2011 Jul 12.
Capturing the right ligand at the right spot: a well-balanced system for non-natural amino acid mutagenesis allows the ligand binding sites of a class II G-protein coupled receptor to be mapped and distinct binding domains to be identified for different ligands in the native environment of mammalian cells.
一个平衡良好的非天然氨基酸诱变系统允许对 II 类 G 蛋白偶联受体的配体结合位点进行作图,并在哺乳动物细胞的天然环境中为不同的配体鉴定不同的结合域。