在哺乳动物细胞中掺入 G 蛋白偶联受体的光交联剂：配体比较。
Photo-cross-linkers incorporated into G-protein-coupled receptors in mammalian cells: a ligand comparison.
机构信息
Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
出版信息
Angew Chem Int Ed Engl. 2011 Aug 22;50(35):8077-81. doi: 10.1002/anie.201102646. Epub 2011 Jul 12.
Abstract
Capturing the right ligand at the right spot: a well-balanced system for non-natural amino acid mutagenesis allows the ligand binding sites of a class II G-protein coupled receptor to be mapped and distinct binding domains to be identified for different ligands in the native environment of mammalian cells.
摘要
在正确的位置捕获正确的配体
一个平衡良好的非天然氨基酸诱变系统允许对 II 类 G 蛋白偶联受体的配体结合位点进行作图，并在哺乳动物细胞的天然环境中为不同的配体鉴定不同的结合域。
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