Department of Life Science, Division of Molecular and Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Proteomics. 2011 Aug;11(16):3424-9. doi: 10.1002/pmic.201000212.
Pseudomonas aeruginosa, an opportunistic human bacterial pathogen, constitutively secretes outer membrane vesicles (OMVs) into the extracellular milieu. Although recent progress has revealed that OMVs are essential for pathogenesis of P. aeruginosa, their proteins have not been comprehensively analyzed so far. In this study, we identified 338 vesicular proteins with high confidence by five separate LC-MS/MS analyses. This global proteome profile provides a basis for future studies to elucidate the pathological functions of OMVs from P. aeruginosa.
铜绿假单胞菌是一种机会性人类细菌病原体，它会将外膜囊泡（OMVs）持续分泌到细胞外环境中。尽管最近的研究进展揭示了 OMVs 对铜绿假单胞菌的发病机制至关重要，但迄今为止，它们的蛋白质尚未得到全面分析。在这项研究中，我们通过五次独立的 LC-MS/MS 分析，高置信度地鉴定了 338 种囊泡蛋白。这个全面的蛋白质组图谱为未来研究阐明铜绿假单胞菌 OMVs 的病理功能提供了基础。
