非水毛细管电泳法测定麦冬中薯蓣皂苷元与鲁斯可皂苷元的含量
[Determination of diosgenin and ruscogenin in Radix Ophiopogonis by nonaqueous capillary electrophoresis].
作者信息
Huang Bao-mei, Yao Cheng-wei, Bian Qing-quan, Wang Zhi-guo, Mo Jin-yuan
机构信息
Chemical Department of Mianyang Normal University, Mianyang 621000, China.
出版信息
Yao Xue Xue Bao. 2011 Apr;46(4):443-6.
Nonaqueous capillary electrophoresis is used for the determination of the contents of diosgenin and ruscogenin in Radix Ophiopogonis. The operating buffer was composed of 20 mmol x L(-1) Na2B4O7-HCl (pH 7.61) in 70% methanol. The applied voltage was 25 kV and detection potential was at +0.70 V. With these conditions, the components were successfully separated. The content of diosgenin in Radix Ophiopogonis was 0.018 mg x g(-1) and ruscogenin was 0.008 mg x g(-1). The average recoveries of diosgenin and ruscogenin were 102% and 99.2%, respectively. A new method of the quality control of diosgenin and ruscogenin in Radix Ophiopogonis is provided.
非水毛细管电泳法用于测定麦冬中薯蓣皂苷元与鲁斯可皂苷元的含量。运行缓冲液由70%甲醇中的20 mmol·L⁻¹ Na₂B₄O₇ - HCl（pH 7.61）组成。施加电压为25 kV，检测电位为 +0.70 V。在此条件下，各成分成功分离。麦冬中薯蓣皂苷元的含量为0.018 mg·g⁻¹，鲁斯可皂苷元的含量为0.008 mg·g⁻¹。薯蓣皂苷元和鲁斯可皂苷元的平均回收率分别为102%和99.2%。提供了一种麦冬中薯蓣皂苷元和鲁斯可皂苷元质量控制的新方法。
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