Zekanowska Ewa, Boinska Joanna, Kwapisz Justyna, Kedziora-Kornatowska Kornelia, Porzych Katarzyna, Ratajczak Marta
Katedra Patofizjologii, Zakład Zaburzeń, Hemostazy Collegium Medicum im. L. Rydygiera w Bydgoszczy, UMK w Toruniu.
Przegl Lek. 2011;68(2):82-6.
Hepcidin is a newly discovered iron metabolism regulator. It decreases iron absorption from the duodenal enterocytes and inhibits its release from the reticuloendothelial macrophages. The experiments on hepcidin and its precursor--prohepcidin seem very promising and underline the need to establish the reference intervals for these proteins and define the effects of age and sex on their concentrations.
The aim of this study was to assess the impact of age and sex on serum prohepcidin concentration in healthy adults.
The study group consisted of 79 healthy volunteers (F-29, M-50) between 18 to 88 years of age. Healthy adults were divided into three groups according to age: group 1-33 healthy volunteers aged between 18-36, group 2-20 healthy volunteers aged between 38-53, group 3- 26 healthy volunteers aged between 58-88. Prohepcidin and ferritin concentrations were determined by enzyme-linked immunosorbent assays (ELISA). Serum iron concentration was measured on Architect c8000 System.
In this work, we demonstrated that prohepcidin concentrations were significantly higher in group 3 (Me = 292,2 ng/ ml) in comparison with group 1 (Me = 150.7 ng/ml; p < 0.000001) or group 2 (Me = 118.1 ng/ml; p < 0.000001). We observed a positive correlation between age and prohepcidin concentration (R = 0.38; p = 0.001). The oldest group of volunteers also revealed significantly higher levels of ferritin and lower levels of serum iron in comparison with two groups of younger adults. Females showed significantly higher level of prohepcidin (Me = 226.51 ng/ml) than males (Me = 142.6 ng/ml; p = 0.01). We observed that females under 50 years of age had significantly higher levels of prohepcidin (Me = 290.9 ng/ml) in comparison with younger females (Me = 150.7 ng/ml; p = 0.0001), similar correlation was noticed in males (212.3 ng/ml vs 128.3 ng/ml; p = 0.007).
Prohepcidin concentration increased significantly with age and was significantly higher in women than men. The results of our study show that serum prohepcidin concentration is age and sex dependent.
铁调素是一种新发现的铁代谢调节剂。它可降低十二指肠肠细胞对铁的吸收，并抑制其从网状内皮巨噬细胞的释放。关于铁调素及其前体——前铁调素的实验似乎很有前景，并强调了建立这些蛋白质的参考区间以及确定年龄和性别对其浓度影响的必要性。
本研究旨在评估年龄和性别对健康成年人血清前铁调素浓度的影响。
研究组由79名年龄在18至88岁之间的健康志愿者组成（女性29名，男性50名）。健康成年人根据年龄分为三组：第1组——33名年龄在18至36岁之间的健康志愿者，第2组——20名年龄在38至53岁之间的健康志愿者，第3组——26名年龄在58至88岁之间的健康志愿者。采用酶联免疫吸附测定法（ELISA）测定前铁调素和铁蛋白浓度。在Architect c8000系统上测量血清铁浓度。
在本研究中，我们发现第3组的前铁调素浓度（中位数 = 292.2 ng/ml）显著高于第1组（中位数 = 150.7 ng/ml；p < 0.000001）或第2组（中位数 = 118.1 ng/ml；p < 0.000001）。我们观察到年龄与前铁调素浓度呈正相关（R = 0.38；p = 0.001）。与两组较年轻的成年人相比，年龄最大的志愿者组的铁蛋白水平也显著更高，血清铁水平更低。女性的前铁调素水平（中位数 = 2
