Department of Infectious Disease, E-DA Hospital/I-Shou University Division of Infectious Diseases, Kaohsiung, Taiwan.
Microbiol Immunol. 2011 Sep;55(9):616-24. doi: 10.1111/j.1348-0421.2011.00365.x.
In this study, it was demonstrated, by using agar diffusion tests and a Transwell system, that Burkholderia multivorans NKI379 has an antagonistic effect against the growth of B. pseudomallei. Bacterial representatives were isolated from agricultural crop soil and mixed to construct a partial bacterial community structure that was based on the results of reproducible patterns following PCR-denaturing gradient gel electrophoresis analysis of total soil chromosomes. The antagonistic effect of B. multivorans on B. pseudomallei was observed in this imitate community. In a field study of agricultural crop soil, the presence of B. pseudomallei was inversely related to the presence of the antagonistic strains B. multivorans or B. cenocepacia. B. multivorans NKI379 can survive in a broader range of pH, temperatures and salt concentrations than B. pseudomallei, suggesting that B. multivorans can adapt to extreme environmental changes and therefore predominates over B. pseudomallei in natural environments.
在这项研究中，通过琼脂扩散试验和 Transwell 系统，证明了伯克霍尔德氏菌 NKI379 对类鼻疽伯克霍尔德氏菌的生长具有拮抗作用。细菌代表从农业作物土壤中分离出来，并混合构建了一个部分细菌群落结构，该结构基于总土壤染色体 PCR-变性梯度凝胶电泳分析结果后的可重复模式。在这个模拟群落中观察到伯克霍尔德氏菌对类鼻疽伯克霍尔德氏菌的拮抗作用。在农业作物土壤的田间研究中，类鼻疽伯克霍尔德氏菌的存在与拮抗菌株伯克霍尔德氏菌或洋葱伯克霍尔德氏菌的存在呈负相关。与类鼻疽伯克霍尔德氏菌相比，伯克霍尔德氏菌 NKI379 可以在更宽的 pH 值、温度和盐浓度范围内存活，这表明伯克霍尔德氏菌可以适应极端的环境变化，因此在自然环境中占主导地位。
