多食伯克霍尔德菌CCA53的鉴定与特性分析
Identification and characterization of Burkholderia multivorans CCA53.
作者信息
Akita Hironaga, Kimura Zen-Ichiro, Yusoff Mohd Zulkhairi Mohd, Nakashima Nobutaka, Hoshino Tamotsu
机构信息
Research Institute for Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 3-11-32 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-0046, Japan.
Department of Civil and Environmental Engineering, National Institute of Technology, Kure College, 2-2-11 Aga-minami, Kure, Hiroshima, 737-8506, Japan.
出版信息
BMC Res Notes. 2017 Jul 6;10(1):249. doi: 10.1186/s13104-017-2565-1.
OBJECTIVE
A lignin-degrading bacterium, Burkholderia sp. CCA53, was previously isolated from leaf soil. The purpose of this study was to determine phenotypic and biochemical features of Burkholderia sp. CCA53.
RESULTS
Multilocus sequence typing (MLST) analysis based on fragments of the atpD, gltD, gyrB, lepA, recA and trpB gene sequences was performed to identify Burkholderia sp. CCA53. The MLST analysis revealed that Burkholderia sp. CCA53 was tightly clustered with B. multivorans ATCC BAA-247. The quinone and cellular fatty acid profiles, carbon source utilization, growth temperature and pH were consistent with the characteristics of B. multivorans species. Burkholderia sp. CCA53 was therefore identified as B. multivorans CCA53.
目的
先前从落叶土壤中分离出一种木质素降解细菌——伯克霍尔德菌属CCA53菌株。本研究旨在确定伯克霍尔德菌属CCA53菌株的表型和生化特征。
结果
基于atpD、gltD、gyrB、lepA、recA和trpB基因序列片段进行多位点序列分型（MLST）分析，以鉴定伯克霍尔德菌属CCA53菌株。MLST分析表明，伯克霍尔德菌属CCA53菌株与多食伯克霍尔德菌ATCC BAA - 247紧密聚类。其醌类和细胞脂肪酸谱、碳源利用情况、生长温度和pH值均与多食伯克霍尔德菌的特征一致。因此，伯克霍尔德菌属CCA53菌株被鉴定为多食伯克霍尔德菌CCA53。
Zotero 插件