Department of Basic Oncology, Institute of Oncology, Hacettepe University, 06100 Sihhiye, Ankara, Turkey.
Cell Immunol. 2011;271(1):134-40. doi: 10.1016/j.cellimm.2011.06.016. Epub 2011 Jun 24.
Maturation of macrophages is influenced by the composition of surrounding microenvironment. Expression of CMKLR1, the receptor for chemerin, is potentially associated with the differentiation status of macrophages. In this study, CMKLR1 was determined on peritoneal and tumor-infiltrating macrophages. CMKLR1 expression was found to be associated with the fibroblast-assisted maturation of J744A.1 monocyte/macrophage cells in the co-cultures established to model tumor microenvironment, whereas the presence of tumor cells was able to upregulate CMKLR1 expression independent of macrophage maturation. In addition, macrophages cultured with tumor cells or in tumor cell-conditioned media responded to recombinant chemerin(17-156) peptide and increased the expression of proinflammatory IL-1β, TNF-α and IL-12 p40 cytokines. The native form of chemerin (prochemerin) supplied by fibroblasts did not induce a functional response. These observations may indicate a potential role for chemerin and CMKLR1 in the regulation of inflammatory responses in the tumor microenvironment.
巨噬细胞的成熟受周围微环境组成的影响。Chemerin 的受体 CMKLR1 的表达可能与巨噬细胞的分化状态有关。在本研究中，检测了腹膜和肿瘤浸润巨噬细胞中的 CMKLR1。CMKLR1 的表达与在模拟肿瘤微环境的共培养物中，成纤维细胞辅助 J744A.1 单核/巨噬细胞分化有关，而肿瘤细胞的存在能够独立于巨噬细胞成熟而上调 CMKLR1 的表达。此外，与肿瘤细胞共培养或在肿瘤细胞条件培养基中培养的巨噬细胞对重组 Chemerin(17-156)肽作出反应，增加促炎细胞因子 IL-1β、TNF-α 和 IL-12 p40 的表达。成纤维细胞提供的天然形式的 Chemerin（prochemerin）不能诱导功能反应。这些观察结果可能表明 Chemerin 和 CMKLR1 在肿瘤微环境中调节炎症反应方面具有潜在作用。
