γ-氨基丁酸A受体介导的犬回结肠交界处非肾上腺素能非胆碱能神经元的刺激作用。
GABAA receptor-mediated stimulation of non-adrenergic non-cholinergic neurones in the dog ileocolonic junction.
作者信息
Boeckxstaens G E, Pelckmans P A, Rampart M, Ruytjens I F, Verbeuren T J, Herman A G, Van Maercke Y M
机构信息
Division of Gastroenterology, Faculty of Medicine, University of Antwerp, Antwerpen-Wilrijk, Belgium.
出版信息
Br J Pharmacol. 1990 Oct;101(2):460-4. doi: 10.1111/j.1476-5381.1990.tb12730.x.
Abstract
- The inhibitory effects of gamma-aminobutyric acid (GABA), the GABAA receptor agonist homotaurine and the GABAB receptor agonist (+/-)-baclofen were investigated on circular muscle strips of the dog terminal ileum and ileocolonic junction. 2. In the presence of atropine, GABA and homotaurine induced concentration-dependent relaxations, similar to the non-adrenergic non-cholinergic (NANC)-mediated relaxations evoked by electrical stimulation or by acetylcholine. The ileocolonic junction was more sensitive to GABA and homotaurine than the ileum. (+/-)-Baclofen had no effect. Cross desensitization only occurred between GABA and homotaurine. 3. The GABAA receptor antagonist bicuculline shifted the concentration-response curves to GABA and homotaurine to the right. The maximal relaxation to GABA remained unaffected. 4. GABA-induced relaxations were not inhibited by timolol, guanethidine, domperidone, hexamethonium and desensitization to ATP, but were abolished by tetrodotoxin. 5. Bicuculline, and pretreatment with GABA or (+/-)-baclofen had no effect on the NANC-evoked relaxations to electrical stimulation and acetylcholine. 6. In conclusion, GABA stimulates GABAA receptors located on inhibitory NANC neurones in the dog ileocolonic junction. Our results suggest that it is unlikely that GABA is the final inhibitory NANC neurotransmitter.
摘要
- 研究了γ-氨基丁酸（GABA）、GABAA受体激动剂高牛磺酸和GABAB受体激动剂（±）-巴氯芬对犬回肠末端和回结肠连接处环形肌条的抑制作用。2. 在阿托品存在的情况下，GABA和高牛磺酸诱导浓度依赖性舒张，类似于电刺激或乙酰胆碱诱发的非肾上腺素能非胆碱能（NANC）介导的舒张。回结肠连接处对GABA和高牛磺酸比回肠更敏感。（±）-巴氯芬无作用。交叉脱敏仅发生在GABA和高牛磺酸之间。3. GABAA受体拮抗剂荷包牡丹碱将GABA和高牛磺酸的浓度-反应曲线向右移动。对GABA的最大舒张不受影响。4. GABA诱导的舒张不受噻吗洛尔、胍乙啶、多潘立酮、六甲铵和对ATP脱敏的抑制，但被河豚毒素消除。5. 荷包牡丹碱以及用GABA或（±）-巴氯芬预处理对NANC诱发的对电刺激和乙酰胆碱的舒张无作用。6. 总之，GABA刺激犬回结肠连接处抑制性NANC神经元上的GABAA受体。我们的结果表明，GABA不太可能是最终的抑制性NANC神经递质。
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