Laboratory of Reproduction Biotechnology, Science and Technology Animal Institute, Universidad Politécnica de Valencia, Valencia, Spain.
Theriogenology. 2011 Oct 1;76(6):1120-5. doi: 10.1016/j.theriogenology.2011.05.020. Epub 2011 Jul 12.
The study evaluated a seminal effect on the ability to induce ovulation of a synthetic GnRH analogue, buserelin acetate, administered by vaginal mucosa in rabbit does. In a first experiment, 751 receptive nulliparous and multiparous non-lactating does were randomly assigned to groups of different seminal doses (6, 12, 24, 50, and 100 million total sperm in 0.5 mL). All seminal doses contained 5 μg of buserelin acetate to induce ovulation by vaginal mucosa absorption. Two hundred and six does from 751 were laparoscopized at 12(th) days of gestation to evaluate ovulation induction, ovulation rate and implanted embryos, while pregnancy rate and total and live born were noted in all females. Results showed that the pregnancy rate was significantly affected by the seminal dose used (0.82 vs 0.72, 0.50, and 0.45, for 6, 24, 50, and 100 million of spermatozoa, respectively). Data from laparoscopized does showed significant differences between the group of 6 and 50 million sperm dose in the ovulation induction and consequently in the pregnancy rate (0.79 vs 0.52, 0.79 vs 0.48, respectively). Does from all groups had similar implanted embryos and litter sizes irrespective of seminal dose used. In a second experiment, inseminations were done without spermatozoa, 0.5 mL of two dilutions of seminal plasma (1/4 and 1/20) with 5 μg of buserelin acetate were introduced into vagina from 71 receptive females and its results were compared to a control group (35 does) induced to ovulate with 1 μg of buserelin acetate administered intramuscularly. Only 40% of females from 1/4 plasma dilution group became to ovulate. Consequently, the dilution rate of seminal plasma may reduce the availability rate of the GnRH analogue and the concentration needed to provoke the ovulation induction.
该研究评估了通过阴道黏膜给予合成 GnRH 类似物布舍瑞林醋酸酯对诱导排卵的基本作用，用于兔。在第一个实验中，751 只接受的初产和经产非哺乳期母兔被随机分配到不同精液剂量组（0.5 毫升中含有 6、12、24、50 和 100 百万个总精子）。所有精液剂量均含有 5μg 的布舍瑞林醋酸酯，通过阴道黏膜吸收诱导排卵。751 只母兔中的 206 只在妊娠第 12 天进行腹腔镜检查，以评估排卵诱导、排卵率和着床胚胎，而所有母兔的妊娠率和总产仔数和活产仔数均被记录。结果表明，妊娠率受使用的精液剂量显著影响（0.82 对 0.72、0.50 和 0.45，分别为 6、24、50 和 100 百万个精子）。腹腔镜检查母兔的数据显示，在排卵诱导和妊娠率方面，6 百万和 50 百万精子剂量组之间存在显著差异（0.79 对 0.52、0.79 对 0.48）。无论使用的精液剂量如何，所有组的母兔着床胚胎和产仔数相似。在第二个实验中，在 71 只接受的母兔阴道内注入不含精子的两种精液血浆稀释液（1/4 和 1/20），每个剂量为 0.5 毫升，其中含有 5μg 的布舍瑞林醋酸酯，其结果与对照组（35 只母兔）进行比较，对照组通过肌肉注射 1μg 的布舍瑞林醋酸酯诱导排卵。只有 40%的 1/4 血浆稀释组母兔排卵。因此，精液血浆的稀释率可能降低 GnRH 类似物的可用性率和诱导排卵所需的浓度。
