Viudes-de-Castro M P, Mocé E, Lavara R, Marco-Jiménez F, Vicente J S
Centro de Investigación y Tecnología Animal-Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono La Esperanza, Segorbe, Castellón, Spain.
Centro de Investigación y Tecnología Animal-Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Polígono La Esperanza, Segorbe, Castellón, Spain.
Theriogenology. 2014 Jun;81(9):1223-8. doi: 10.1016/j.theriogenology.2014.02.003. Epub 2014 Feb 13.
Ovulation induction in artificially inseminated rabbits by adding GnRH synthetic analogues in the seminal doses is a welfare-orientated method to induce ovulation in rabbits and could have some advantages in field practice. This study was conducted to determine the effect of male genotype on the aminopeptidase activity in rabbit seminal plasma and the effects of dilution rate of semen on availability and reproductive performance when buserelin acetate is added to the seminal dose. To study the aminopeptidase activity, 12 mature bucks belonging to a paternal line and 12 from a maternal line were used. The bucks from the paternal line were used to study the effect of dilution rate on the availability of buserelin acetate after 2 hours of dilution and on the reproductive performance of the doses after artificial insemination of 389 commercial crossbreed does. Aminopeptidase activity in seminal plasma is dependent on the male genotype. The paternal line resulted 27% more aminopeptidase activity than the maternal line (P < 0.05). On the other hand, semen diluted 1:20 exhibited a marked increase in the availability of buserelin acetate and the fertility in this group was significantly higher than females from dilution rate 1:5 group, which showed similar results to that of the negative control group (does inseminated with semen diluted 1:20 in non-GnRH-supplemented extender). We conclude that the bioavailability of buserelin acetate when added to the seminal dose appears to be determined by the activity of the existing aminopeptidases and is consequently affected by the dilution rate used to prepare the artificial insemination doses.
在人工授精的家兔精液剂量中添加促性腺激素释放激素（GnRH）合成类似物来诱导排卵，是一种以福利为导向的家兔排卵诱导方法，在实际应用中可能具有一些优势。本研究旨在确定雄性基因型对家兔精浆中氨肽酶活性的影响，以及当在精液剂量中添加醋酸布舍瑞林时，精液稀释率对其有效性和繁殖性能的影响。为了研究氨肽酶活性，使用了12只属于父系的成年雄兔和12只属于母系的成年雄兔。父系雄兔的精液用于研究稀释率对稀释2小时后醋酸布舍瑞林有效性的影响，以及对389只商品杂交母兔人工授精后精液剂量繁殖性能的影响。精浆中的氨肽酶活性取决于雄性基因型。父系的氨肽酶活性比母系高27%（P<0.05）。另一方面，精液按1:20稀释后，醋酸布舍瑞林的有效性显著增加，该组的受孕率明显高于1:5稀释率组的母兔，后者的结果与阴性对照组（用未添加GnRH的稀释液按1:20稀释精液进行人工授精的母兔）相似。我们得出结论，当在精液剂量中添加醋酸布舍瑞林时，其生物利用度似乎由现有的氨肽酶活性决定，因此会受到制备人工授精剂量时所用稀释率的影响。
