Haluzová Ivana, Modrá Helena, Blahová Jana, Havelková Marcela, Siroká Zuzana, Svobodová Zdeňka
Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
Interdiscip Toxicol. 2011 Jun;4(2):85-9. doi: 10.2478/v10102-011-0015-9.
Markers of xenobiotic metabolization (cytochrome P450, ethoxyresorufin-O-deethylase, glutathione and glutathione-S-transferase) were investigated in the liver of the common carp Cyprinus carpio after 28-day exposure to different pesticide formulations.The fish exposed to herbicide Sencor 70 WG (metribuzin 700 g/kg) of 0.25 and 2.5 mg/l showed no change in cytochrome P450 and activity of ethoxyresorufin-O-deethylase when compared to control.Successor 600 (pethoxamid 600 g/l) of 0.06; 0.22 and 0.60 mg/l did not affect either cytochrome P450 or the activity of ethoxyresorufin-O-deethylase. However, in fish exposed to Successor 600 of 0.22 and 0.60 mg/l, there was a rise in glutathione and in the activity of glutathione-S-transferase (p<0.05), with Spearman's correlation r = 0.23 at p<0.05.Spartakus (prochloraz 450 g/l) of 0.36 and 1.08 mg/l induced cytochrome P450 and ethoxyresorufin-O-deethylase (p<0.05), with Spearman's correlation r=0.49 at p<0.01. Glutathione increased in fish exposed to 1.08 mg/l (p<0.05), the activity of glutathione-S-transferase rose (p<0.05) in all concentrations tested (0.108; 0.36 and 1.08 mg/l). Spearman's correlation between glutathione and GST was r=0.38; p<0.01).The obtained data contribute to a better understanding of detoxification of the selected xenobitics in fish. Although biomarkers of the first phase of metabolization are considered to be more sensitive, our results indicate higher sensitivity of the second phase biomarkers.
在将鲤鱼（Cyprinus carpio）暴露于不同农药制剂28天后，研究了其肝脏中异源物质代谢的标志物（细胞色素P450、乙氧芴香豆素 - O - 脱乙基酶、谷胱甘肽和谷胱甘肽 - S - 转移酶）。与对照组相比，暴露于浓度为0.25和2.5 mg/l的除草剂赛克津70 WG（嗪草酮700 g/kg）的鱼类，其细胞色素P450和乙氧芴香豆素 - O - 脱乙基酶的活性没有变化。浓度为0.06、0.22和0.60 mg/l的后继者600（稗草胺600 g/l）对细胞色素P450或乙氧芴香豆素 - O - 脱乙基酶的活性均无影响。然而，在暴露于浓度为0.22和0.60 mg/l后继者600的鱼类中，谷胱甘肽和谷胱甘肽 - S - 转移酶的活性有所升高（p<0.05），Spearman相关性r = 0.23，p<0.05。浓度为0.36和1.08 mg/l的斯巴达克（咪鲜胺450 g/l）诱导了细胞色素P450和乙氧芴香豆素 - O - 脱乙基酶（p<0.05），Spearman相关性r = 0.49，p<0.01。暴露于1.08 mg/l的鱼类中谷胱甘肽增加（p<0.05），在所有测试浓度（0.108、0.36和1.08 mg/l）下谷胱甘肽 - S - 转移酶的活性均升高（p<0.05）。谷胱甘肽与谷胱甘肽 - S - 转移酶之间的Spearman相关性为r = 0.38，p<0.01。所获得的数据有助于更好地理解鱼类中所选异源物质的解毒作用。尽管第一阶段代谢的生物标志物被认为更敏感，但我们的结果表明第二阶段生物标志物具有更高的敏感性。
