亚慢性嗪草酮暴露对鲤鱼（鲤属）的影响。
Effects of subchronic metribuzin exposure on common carp (Cyprinus carpio).
作者信息
Modra Helena, Haluzova Ivana, Blahova Jana, Havelkova Marcela, Kruzikova Kamila, Mikula Premysl, Svobodova Zdenka
机构信息
Department of Veterinary Public Health and Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic.
出版信息
Neuro Endocrinol Lett. 2008 Oct;29(5):669-74.
OBJECTIVES
Effects of metribuzin on biochemical and haematological indices, induction of specific biomarkers and impacts on biometric parameters of Cyprinus carpio were investigated for subchronic conditions.
METHODS
Juvenile fish were exposed to 0.175 mg.L-1 or 1.75 mg.L-1 metribuzin for 28 days. Haematological indices were assessed using unified methods of haematological examination in fish. Biochemical indices were determined by biochemical analyzer, hepatic vitellogenin content was estimated by direct sandwich ELISA. Cytochrome P450 concentration and ethoxyresorufin O-deethylase activity were measured in liver spectrophotometrically and spectrofluorimetrically, respectively.
RESULTS
Increased haematocrit and RBC count were found in fish exposed to 1.75 mg.L-1 metribuzin compared to control fish (p<0.05). Other indices contents and biomarkers levels were not significantly changed by either concentration of metribuzin.
CONCLUSION
Sublethal metribuzin pollution may have adverse impacts on haematological parameters in common carp.
目的
研究嗪草酮在亚慢性条件下对鲤鱼生化和血液学指标、特定生物标志物的诱导作用以及对生物特征参数的影响。
方法
将幼鱼暴露于0.175毫克/升或1.75毫克/升的嗪草酮中28天。使用鱼类血液学检查的统一方法评估血液学指标。通过生化分析仪测定生化指标，通过直接夹心酶联免疫吸附测定法估计肝脏卵黄蛋白原含量。分别用分光光度法和荧光分光光度法测定肝脏中的细胞色素P450浓度和乙氧基试卤灵O-脱乙基酶活性。
结果
与对照鱼相比，暴露于1.75毫克/升嗪草酮的鱼的血细胞比容和红细胞计数增加（p<0.05）。嗪草酮的任一浓度均未显著改变其他指标含量和生物标志物水平。
结论
亚致死剂量的嗪草酮污染可能对鲤鱼的血液学参数产生不利影响。
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