College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China; Animal Health Supervision Institute of Heilongjiang Province, 243 Haping Road, Xiangfang District, Harbin 150069, PR China.
College of Animal Science and Technology, Northeast Agricultural University, 59 Mucai Street, Harbin 150030, PR China.
Chemosphere. 2014 Jun;104:244-50. doi: 10.1016/j.chemosphere.2014.01.002. Epub 2014 Feb 14.
Atrazine (ATR) and chlorpyrifos (CPF), widely used in agriculture, have resulted in a series of toxicological and environmental problems. We investigated the activities of the biotransformation enzymes ethoxyresorufin-O-deethylase (EROD) and pentoxyresorufin-O-deethylase (PROD), total cytochrome P450 (CYP), CYP1A mRNA level and level of tissue ATR, CPF, and their metabolites in the liver of common carp (Cyprinus carpio L.) after a 40-d exposure to CPF and ATR, alone or in combination, and a 20-d recovery. In the present study, juvenile common carp was exposed to ATR (at concentrations of 4.28, 42.8 and 428 μg L(-1)), CPF (1.16, 11.6 and 116 μg L(-1)), and ATR/CPF mixture (at concentrations of 1.13, 11.3 and 113 μg L(-1)). A general increasing trend for the activity of the biotransformation enzymes (EROD and PROD), CYP and CYP1A mRNA level was observed in the liver of common carp exposed to ATR, CPF and the ATR/CPF mixture. In addition, ATR, CPF, and their metabolites demonstrated a high accumulation in the liver. These results demonstrated that the CYP system in fish could be used as a biomarkers in evaluating the impact of ATR and CPF exposure on the common carp.
阿特拉津（ATR）和毒死蜱（CPF）广泛应用于农业，导致了一系列毒理学和环境问题。我们研究了生物转化酶对氧磷酶（EROD）和对氧磷酶（PROD）、总细胞色素 P450（CYP）、CYP1A mRNA 水平以及鲤鱼（Cyprinus carpio L.）肝脏中 ATR、CPF 及其代谢物水平的活性，在单独或联合暴露于 CPF 和 ATR 40 天和 20 天恢复期后。在本研究中，幼年鲤鱼暴露于 ATR（浓度为 4.28、42.8 和 428μg/L）、CPF（1.16、11.6 和 116μg/L）和 ATR/CPF 混合物（浓度为 1.13、11.3 和 113μg/L）。暴露于 ATR、CPF 和 ATR/CPF 混合物的鲤鱼肝脏中，生物转化酶（EROD 和 PROD）、CYP 和 CYP1A mRNA 水平的活性呈普遍升高趋势。此外，ATR、CPF 和它们的代谢物在肝脏中高度积累。这些结果表明，鱼类中的 CYP 系统可以作为评估 ATR 和 CPF 暴露对鲤鱼影响的生物标志物。
